HSPB8 antibody

Catalog No. ABIN6656215

Supplier Product No.: 200-301-f53

Product Details anti-HSPB8 Antibody

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPB8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Supplier Product No.: 200-301-f53

Supplier: Rockland

Purpose: HSP22 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp22 from human, mouse, and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Hsp22 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Hsp22 Antibody was produced in mice by repeated immunizations raised against a his-tagged human recombinant hsp22.

Clone: 3C12-H11

Isotype: IgG1 kappa

Application Details

Application Notes:

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:2000

Comment:

Anti-Hsp22 Antibody is tested for use in WB, IHC and IF. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HSPB8

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Alternative Name: Hsp22 (HSPB8 Products)

Background:

Synonyms: Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, H11, Heat shock 22 kDa protein 8, HMN2, HSB8, HspB8, Heat shock protein beta-8, E2IG1, HSP22, E2-induced gene 1 protein, Protein kinase H11

Background: Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27. HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy. Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).

Gene Name: HSPB8

Gene ID: 26353

NCBI Accession: NP_055180

UniProt: Q9UJY1