GFAP R416WT antibody (AA 411-422) (HRP)

Catalog No. ABIN6656180

Product Details anti-GFAP R416WT Antibody (HRP)

Target: GFAP R416WT

Binding Specificity: AA 411-422

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP R416WT antibody is conjugated to HRP

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-GFAP R416WT Antibody was purified from concentrated tissue culture supernate by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with rat and mouse based on 100% homology with the immunizing sequence. No cross-reactivity with other GFAP mutant proteins can be expected.

Immunogen:

Immunogen: Anti-GFAP R416WT Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids 411-422 (KTVEMRDGEVIK) of human GFAP.

Immunogen Type: Peptide

Clone: S206B-9

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-GFAP R416WT HRP Conjugated Antibody is suitable for use in Western blot, Immunohistochemistry, and Immunocytochemistry. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for GFAP R416WT

Target: GFAP R416WT

Abstract: GFAP R416WT Products

Background:

Synonyms: Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, GFAP antibody

Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia. Anti-GFAP is ideal for investigators involved in Neuroscience Research, including Alexander Disease, Oligodendroglioma, Cytoskleton Remolding Neurofilaments and PIP3/AKT Signaling.

Gene Name: GFAP

Gene ID: 2670

UniProt: P14136