FKBP5 antibody

Catalog No. ABIN6656088

Supplier Product No.: 200-301-f28

Product Details anti-FKBP5 Antibody

Target: FKBP5 (FK506 Binding Protein 5 (FKBP5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FKBP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 200-301-f28

Supplier: Rockland

Purpose: FKBP51 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with FKBP51 from Canine, Hamster, Human, Mouse, Rat, and Rabbit based on 100 % homology with the immunizing sequence.

Purification: Anti-FKBP51 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: FKBP51 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the residues of human FKBP51.

Clone: Hi51B

Isotype: IgG1

Application Details

Application Notes:

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:2000

Comment:

Anti-FKBP51 Antibody is tested for IF and WB. Expect a band approximately ~51kDa protein representing FKBP51 in cell lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FKBP5

Target: FKBP5 (FK506 Binding Protein 5 (FKBP5))

Alternative Name: FKBP51 (FKBP5 Products)

Background:

Synonyms: AIG6, FK506 binding protein 5, FKBP5, FKBP54, FKBP51, Hsp90 binding immunophilin, p54, Pplase, Ptg10, Rotamase, T cell FK506 binding protein antibody

Background: Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones. One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins. FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex. The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90. Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics . FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids.

Gene Name: FKBP5

Gene ID: 2289

NCBI Accession: NP_001139247

UniProt: Q13451