UBE2M antibody (AA 169-180)

Catalog No. ABIN6656076

Supplier Product No.: 600-401-865

Product Details anti-UBE2M Antibody

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Binding Specificity: AA 169-180

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2M antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-865

Supplier: Rockland

Purpose: UBC12 Antibody

Cross-Reactivity (Details): Reactivity occurs against human UBC12 protein.

Purification: UBC12 Antibody is produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region near aa 150-183 of Human UBC12 protein.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:2,000 - 1:10,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Comment:

UBC12 Antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to UBC12 by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-UBE2M antibody (ABIN6656076)

Liu, Reitsma, Mamrosh, Zhang, Straube, Deshaies: "Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression." in: Molecular cell, Vol. 69, Issue 5, pp. 773-786.e6, (2019) (PubMed).

Kolokoltsova, Domina, Kolokoltsov, Davey, Weaver, Watowich: "Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling." in: Virology, Vol. 377, Issue 1, pp. 133-42, (2008) (PubMed).

Target Details for UBE2M

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Alternative Name: UBC12 (ube2m Products)

Background:

Synonyms: rabbit anti-UBC12 antibody, UBC-12, UBC 12, NEDD-8, NEDD 8, NEDD8 carrier protein antibody, NEDD8 conjugating enzyme Ubc12 antibody, NEDD8 protein ligase antibody, UBE2M antibody, Ubiquitin conjugating enzyme E2 M antibody

Background: UBC12 (also known as Ubiquitin-conjugating enzyme E2M, Ubiquitin-protein ligase M, Ubiquitin carrier protein M, and Nedd8-conjugating enzyme Ubc12) is a member of the E2 ubiquitin-conjugating enzyme family.  The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBC12 is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/cullin.

Gene Name: UBE2M

Gene ID: 9040, 4507791

UniProt: P61081