Hsc70 antibody

Catalog No. ABIN6656068

Supplier Product No.: 200-301-a28

Product Details anti-Hsc70 Antibody

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS)

Supplier Product No.: 200-301-a28

Supplier: Rockland

Purpose: Hsc70 (Hsp73) Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Hsc70 from human, bovine, mouse, rat, C. Elegans

Purification: This Protein G purified monoclonal antibody reacts with human Hsc70 (Hsp73) protein.

Sterility: Sterile filtered

Immunogen: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide corresponding to a region of human Hsc70 (Hsp73) protein.

Clone: N27F3-4

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Comment:

This Protein G purified antibody has been tested for use in western blotting, immunoelectron microscopy, immunohistochemistry and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a doublet band approximately 72/73 kDa in size corresponding to Hsc70 (Hsp73) by western blotting in the appropriate cell lysate or extract.  In general, a 1:1,000 dilution is suggested for most applications and is suitable to detect Hsc70 (Hsp73) in 20 μg of heat shocked HeLa cell lysate by western blotting.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

References for anti-Hsc70 antibody (ABIN6656068)

Kim, Kim, Lee, Eom, Kim, Park, Jeong, Lee: "Transcription Factor TonEBP Stimulates Hyperosmolality-Dependent Arginine Vasopressin Gene Expression in the Mouse Hypothalamus." in: Frontiers in endocrinology, Vol. 12, pp. 627343, (2021) (PubMed).

Ye, Lee, Yoo, Lee, Lee, Kang, Jeong, Park, Lee-Kwon, Choi, Kwon: "TonEBP in dendritic cells mediates pro-inflammatory maturation and Th1/Th17 responses." in: Cell death & disease, Vol. 11, Issue 6, pp. 421, (2021) (PubMed).

Lee, An, Ye, Lee, Yoo, Jeong, Kang, Alfadda, Lim, Park, Lee-Kwon, Kim, Choi, Kwon: "TonEBP/NFAT5 promotes obesity and insulin resistance by epigenetic suppression of white adipose tissue beiging." in: Nature communications, Vol. 10, Issue 1, pp. 3536, (2019) (PubMed).

Lee, Sanada, An, Ye, Lee, Seo, Lee, Lee-Kwon, Küper, Neuhofer, Choi, Kwon: "LPS-induced NFκB enhanceosome requires TonEBP/NFAT5 without DNA binding." in: Scientific reports, Vol. 6, pp. 24921, (2018) (PubMed).

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: Hsc70 Hsp73 (HSPA8 Products)

Background:

Synonyms: Heat shock cognate protein 71- kDa antibody, Heat shock protein 8 antibody, Heat-shock70-KD protein 10, formerly antibody, HSC54 antibody, HSC71 antibody

Background: Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes, hsp70 genes exist as part of a multigene family. Hsp70s are found in most cellular compartments of eukaryotes, including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two-thirds of hsp70s are more conserved than the C-terminal one-third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins, preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization, and protein transport.

Gene Name: HSPA1A

Gene ID: 3312

NCBI Accession: NP_006588

UniProt: P11142