VAMP 1,2 antibody

Catalog No. ABIN6656054

Supplier Product No.: 200-301-e34

Product Details

Target: VAMP 1,2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 200-301-e34

Supplier: Rockland

Purpose: Synaptobrevin (VAMP) Antibody

Cross-Reactivity (Details): Anti-Synaptobrevin (VAMP) Antibody is directed against human synaptobrevin.

Purification: Anti-Synaptobrevin was IgM purified by chromatography from cell culture supernatant.

Immunogen: Anti-Synaptobrevin (VAMP) Monoclonal Antibody was produced in mouse by repeated immunizations with synaptic immunoprecipitate (crude) from human brain.

Clone: SP10

Isotype: IgM

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Anti-Synaptobrevin antibody has been tested in Western Blotting and IHC. Expect a band of approximately 16 kDa in size corresponding to the VAMP proteins in Western blot of rat brain lysate. Anti-Synaptobrevin antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections. Specific conditions for reactivity should be optimized by the end user. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: VAMP 1,2

Alternative Name: VAMP1/2

Background:

Synonyms: Vesicle-associated membrane protein 1

Background: Synaptobrevin (also VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles. Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive defects in Alzheimer's disease.

Gene Name: VAMP1/2

Gene ID: 6843

UniProt: P23763