HSPE1 antibody (C-Term)

Catalog No. ABIN6656049

Supplier Product No.: 200-401-f17

Product Details anti-HSPE1 Antibody

Target: HSPE1 (Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 200-401-f17

Supplier: Rockland

Purpose: CPN10 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Cpn 10 from Human, Mouse, Rat, Bovine, Canine, Guinea pig, Pig, Rabbit, Sheep, and Xenopus based on 100 % homology with the immunizing sequence.

Purification: Anti-Cpn10 Antibody was purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen: CPN10 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the c-terminal region of human Cpn10 synthetic peptide.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-CPN10 Antibody is tested for use in WB, IHC, IF, and IP. Expect a band approximately ~10kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HSPE1

Target: HSPE1 (Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1))

Alternative Name: Cpn10 (HSPE1 Products)

Background:

Synonyms: 10 kDa Chaperonin, Chaperonin 10, Cpn 10, EPF, GROES, Heat Shock 10kD protein1, Hsp10, HSPE1, Early-pregnancy factor

Background: Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10 kDa (Hsp10s). Cpn10 acts as a co-chaperone and interacts with the Hsp60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process. Cpn10 has been located in human platelets, but is also present in human maternal serum. It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy.

Gene Name: HSPE1

Gene ID: 3336

NCBI Accession: NP_002148

UniProt: P61604

Pathways: Positive Regulation of Endopeptidase Activity