SMC1B antibody (pSer957)

Catalog No. ABIN6655981

Product Details anti-SMC1B Antibody

Target: SMC1B (Structural Maintenance of Chromosomes 1B (SMC1B))

Binding Specificity: pSer957

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMC1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Purpose: SMC1 phospho S957 Antibody

Cross-Reactivity (Details): This monoclonal anti-SMC1 antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts.

Purification: This Protein G Purified Mab antibody is directed against human SMC1 and is useful in determining its presence in various assays.

Sterility: Sterile filtered

Immunogen: This antibody was produced from a synthetic peptide corresponding to aa 951-962 of human SMC1 by injection into a balb/c mouse.

Clone: 5D11-G5

Isotype: IgG1 kappa

Application Details

Application Notes:

ELISA_Dilution: 1:20,000 - 1:100,000

Immunohistochemistry_Dilution: 2.5 μg/mL

IF_Microscopy_Dilution: 2.5 μg/mL

Western_Blot_Dilution: 1:100 - 1:2,000

Other: User Optimized

Comment:

Suggested Applications: IF, Multiplex
Protein G Purified Mab anti-SMC1 was tested by ELISA, immunohistochemistry and western blotting against native protein. The antibody reacts with SMC1 from irradiated human and mouse cells.  A 160 kDa band corresponding to phosphorylated human SMC1 is noted in gamma irradiated human and mouse lysates.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-SMC1B antibody (ABIN6655981)

Callén, Jankovic, Wong, Zha, Chen, Difilippantonio, Di Virgilio, Heidkamp, Alt, Nussenzweig, Nussenzweig: "Essential role for DNA-PKcs in DNA double-strand break repair and apoptosis in ATM-deficient lymphocytes." in: Molecular cell, Vol. 34, Issue 3, pp. 285-97, (2009) (PubMed).

Pusapati, Rounbehler, Hong, Powers, Yan, Kiguchi, McArthur, Wong, Johnson: "ATM promotes apoptosis and suppresses tumorigenesis in response to Myc." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 5, pp. 1446-51, (2006) (PubMed).

Kitagawa, Bakkenist, McKinnon, Kastan: "Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway." in: Genes & development, Vol. 18, Issue 12, pp. 1423-38, (2004) (PubMed).

Target Details for SMC1B

Target: SMC1B (Structural Maintenance of Chromosomes 1B (SMC1B))

Alternative Name: SMC1B (SMC1B Products)

Synonyms: SMC1BETA antibody, SMC1L2 antibody, bK268H5 antibody, bK268H5.5 antibody, SMC-1B antibody, SMC1beta antibody, Smc1l2 antibody, structural maintenance of chromosomes 1B antibody, SMC1B antibody, Smc1b antibody

Background:

Synonyms: mouse anti-SMC1 pS957 antibody, Structural maintenance of chromosomes protein 1B antibody, SMC1beta protein antibody, SMC1B antibody, SMC1L2 antibody

Background: Structural maintenance of chromosomes (SMC) proteins play important roles in sister chromatid cohesion, chromosome condensation, sex-chromosome dosage compensation, and DNA recombination and repair (DNA damage). Protein complexes containing heterodimers of the SMC1 and SMC3 proteins have been implicated specifically in both sister chromatid cohesion and DNA recombination.  ATM, a protein kinase belonging to the phosphatidylinositol 3-kinase family that regulates cell cycle checkpoints and DNA recombination and repair, phosphorylates SMC1 protein after ionizing irradiation. ATM protein kinase phosphorylates SMC1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint.  Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM protein kinase substrates NBS1 and BRCA1. These same sites in SMC1 are phosphorylated after treatment with UV irradiation or hydroxyurea in an ATM-independent manner, thus demonstrating that another kinase must be involved in responses to these cellular stresses. Yeast containing hypomorphic mutations in SMC1 and human cells overexpressing SMC1 mutated at both of these phosphorylation sites exhibit decreased survival following ionizing irradiation. These results demonstrate that SMC1 participates in cellular responses to DNA damage and link SMC1 to the ATM protein kinase signal transduction pathway.

Gene Name: SMC1B

Gene ID: 27127

UniProt: Q8NDV3