MED23 antibody (AA 897-916)

Catalog No. ABIN6655801

Product Details anti-MED23 Antibody

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Binding Specificity: AA 897-916

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED23 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Cross-Reactivity: Human, Mouse (Murine)

Purification: This antiserum was processed by delipidation and defibrination and is directed against mouse DRIP130.  This reagent can be used to determine the presence of DRIP130 various immunological assays. Cross-reactivity occurs with human DRIP-130.  Reactivity with DRIP-130 isolated from other species is unknown.

Immunogen:

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to mouse DRIP-130 residues  897-916 (based on human DRIP-130 residue numbering)  crosslinked to KLH.

Immunogen Type: Peptide

Application Details

Application Notes:

Application Note: Anti-DRIP-130 detects a 130 kDa band by immunoblot analysis using a dilution of 1:1,000. For immunoprecipitation a dilution of 1:100 or 1:200 is suggested, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. Optimal titers for applications should be determined by the researcher. Rockland Immunochemical's anti-DRIP130 has been used to detect DRIP130 using nuclear extracts from mouse thymus, spleen, and brain. Unfortunately, we have not tested reactivity on any cell line extracts. The following standard procedure is recommended: separate 10 micrograms of thymus nuclear extract by 7.5 % SDS-PAGE (0.75 to 1 mm thick). Transfer to nitrocellulose without SDS in the transfer buffer (pre-equilibrate the gel in transfer buffer for 30 min prior to transfer). Blocking is performed with 5 % non-fat dry milk in TTBS for 1 hr at room temperature. Incubate anti-DRIP130 antibody at a 1:1,000 for 1 hr at room temperature. Dilute Donkey anti-Rabbit IgG-HRP 1:5,000 and react 1 hr at room temperature. A predominant band at ~130 KDa is detected under these conditions. Control or normal rabbit serum fails to recognize the 130 kDa protein.

Immunoprecipitation Dilution: 1:100 - 1:200

ELISA Dilution: 1:5,000 - 1:25,000

Western Blot Dilution: 1:1,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.1 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References for anti-MED23 antibody (ABIN6655801)

Kasper, Fukuyama, Brindle: "T-cells null for the MED23 subunit of mediator express decreased levels of KLF2 and inefficiently populate the peripheral lymphoid organs." in: PLoS ONE, Vol. 9, Issue 7, pp. e102076, (2015) (PubMed).

Target Details for MED23

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Alternative Name: DRIP130 (MED23 Products)

Synonyms: CG3695 antibody, Dmel\\CG3695 antibody, Gal11/SUR2 antibody, Med23 antibody, Trap150 antibody, Trap150beta antibody, dSUR2 antibody, dTRAP150beta antibody, CRSP3 antibody, MED23 antibody, ARC130 antibody, CRSP130 antibody, CRSP133 antibody, DRIP130 antibody, MRT18 antibody, SUR-2 antibody, SUR2 antibody, 130kDa antibody, 3000002A17Rik antibody, Crsp3 antibody, ESTM7 antibody, Sur2 antibody, X83317 antibody, mKIAA1216 antibody, crsp3 antibody, si:ch211-272f15.2 antibody, zgc:91979 antibody, Mediator complex subunit 23 antibody, mediator complex subunit 23 antibody, mediator of RNA polymerase II transcription subunit 23 antibody, mediator complex subunit 23 L homeolog antibody, Mediator of RNA polymerase II transcription subunit 23 antibody, MED23 antibody, med23 antibody, LOC412332 antibody, Med23 antibody, med23.L antibody, sur-2 antibody

Background:

Synonyms: rabbit anti-DRIP130antibody, Mediator of RNA polymerase II transcription subunit 23, Mediator complex subunit 23, Cofactor required for Sp1, transcriptional activation subunit 3, CRSP complex subunit 3, CRSP3, Transcriptional coactivator CRSP130, ARC130, DRIP130, KIAA1216, SUR2

Background: This antiserum recognizes mouse DRIP-130 (vitamin-D-receptor interacting protein 130), and was tested for use in immunoprecipitation and immunoblotting.  DRIP-130 has been described elsewhere as Sur2, CRSP130, ARC130, NAT140, and TRAP150b.    DRIP-130 is component of a multi-protein complex termed DRIP that interacts with the vitamin D receptor (VDR), together, the DRIP proteins constitute a new cofactor complex.  DRIP binds to several nuclear receptors and mediates ligand-dependent enhancement of transcription by VDR and the thyroid-hormone receptor in cell-free transcription assays. The DRIPs are almost indistinguishable from components of two other cofactor complexes called ARC and TRAP, which are recruited by other types of transcription activators to mediate transactivation on chromatin-assembled templates.  Several DRIP/ARC/TRAP subunits are also components of other potentially related cofactors, such as CRSP, NAT, SMCC and the mouse Mediator, indicating that unique classes of activators may share common sets or subsets of cofactors. The role of nuclear-receptor ligands may, in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes. In humans, interaction with Sur-2 is required for transcription to be activated by the activation domain of a transcription factor of the ETS-family in response to activated mitogen-activated protein (MAP) kinase.

Gene Name: MED23, DRIP130

Gene ID: 9439, 28558074

UniProt: Q9ULK4

Pathways: Regulation of Lipid Metabolism by PPARalpha