Cyclin L2 antibody (AA 309-384)

Catalog No. ABIN6655712

Supplier Product No.: 600-401-878

Product Details anti-Cyclin L2 Antibody

Target: Cyclin L2 (CCNL2)

Binding Specificity: AA 309-384

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin L2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 600-401-878

Supplier: Rockland

Purpose: Cyclin L2 Antibody

Cross-Reactivity (Details): This antibody is specific for human Cyclin L2a and shows negligible reactivity to Cyclin L2b or Cyclin L1 isoforms.

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using polypeptide coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 309-384 of Human Cyclin L2a protein.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: 1:100

ELISA_Dilution: 1:2,000 - 1:10,000

Western_Blot_Dilution: 1:500 - 1:3,000

Other: User Optimized

Comment:

This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 58 kDa in size corresponding to Cyclin L2a by western blotting in the appropriate cell lysate or extract. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Cyclin L2 antibody (ABIN6655712)

Kren, Unger, Abedin, Vogel, Henzler, Ahmed, Trembley: "Preclinical evaluation of cyclin dependent kinase 11 and casein kinase 2 survival kinases as RNA interference targets for triple negative breast cancer therapy." in: Breast cancer research : BCR, Vol. 17, pp. 19, (2015) (PubMed).

Loyer, Trembley, Katona, Kidd, Lahti: "Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).

Target Details for Cyclin L2

Target: Cyclin L2 (CCNL2)

Alternative Name: Cyclin L2 (CCNL2 Products)

Background:

Synonyms: rabbit anti-Cyclin-L2 antibody, Cyclin L2, CCNL2, SB138, Paneth cell-enhanced expression protein, Cyclin Ania-6b, Paneth cell-enhanced expression protein, PCEE, Ania6b

Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively).  Cyclin L2 can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). The Cyclin L2 gene is located approximately 100-200 kb from the PITSLRE/CDK11 protein kinase locus in human (1p36) and in a syngenetic location on mouse chromosome 4q.  Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Gene Name: CCNL2, SB138

Gene ID: 81669, 14585859

UniProt: Q96S94