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Cyclin L2 antibody (AA 309-384)

CCNL2 Reactivity: Human WB, ELISA, IP Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6655712
  • Target See all Cyclin L2 (CCNL2) Antibodies
    Cyclin L2 (CCNL2)
    Binding Specificity
    • 10
    • 7
    • 7
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 309-384
    Reactivity
    • 33
    • 14
    • 12
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 35
    Rabbit
    Clonality
    • 35
    Polyclonal
    Conjugate
    • 18
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Cyclin L2 antibody is un-conjugated
    Application
    • 26
    • 15
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Cross-Reactivity
    Human, Mouse (Murine)
    Cross-Reactivity (Details)
    Cross reactivity with Cyclin L2a protein from rat and chicken (called Cyclin S) is expected, as this sequence shows ~ 80 % homology with the protein from these sourcesCross reactivity with Cyclin L2a homologues from other sources has not been determined.
    Purification
    This product is an affinity purified antibody produced by immunoaffinity chromatography using polypeptide coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This antibody is specific for human Cyclin L2a and shows negligible reactivity to Cyclin L2b or Cyclin L1 isoforms.  A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L2a protein from rat and chicken (called Cyclin S) is expected, as this sequence shows ~ 80% homology with the protein from these sources. Cross reactivity with Cyclin L2a homologues from other sources has not been determined.
    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 309-384 of Human Cyclin L2a protein.

    Immunogen Type: Peptide

    Isotype
    IgG
  • Application Notes

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 58 kDa in size corresponding to Cyclin L2a by western blotting in the appropriate cell lysate or extract. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation.

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:2,000 - 1:10,000

    Western Blot Dilution: 1:500 - 1:3,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    0.01 % (w/v) Sodium Azide

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
  • Kren, Unger, Abedin, Vogel, Henzler, Ahmed, Trembley: "Preclinical evaluation of cyclin dependent kinase 11 and casein kinase 2 survival kinases as RNA interference targets for triple negative breast cancer therapy." in: Breast cancer research : BCR, Vol. 17, pp. 19, (2015) (PubMed).

    Loyer, Trembley, Katona, Kidd, Lahti: "Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).

  • Target
    Cyclin L2 (CCNL2)
    Alternative Name
    Cyclin L2 (CCNL2 Products)
    Synonyms
    CCNL2 antibody, ANIA-6B antibody, CCNM antibody, HCLA-ISO antibody, HLA-ISO antibody, PCEE antibody, SB138 antibody, CCNL1 antibody, 1700010A01Rik antibody, 1810019L15Rik antibody, 2010319M22Rik antibody, AA409936 antibody, AW261738 antibody, Ania-6b antibody, HCLA-150 antibody, Pcee antibody, cyclin L2 antibody, cyclin L2 L homeolog antibody, CCNL2 antibody, ccnl2.L antibody, Ccnl2 antibody
    Background

    Synonyms: rabbit anti-Cyclin-L2 antibody, Cyclin L2, CCNL2, SB138, Paneth cell-enhanced expression protein, Cyclin Ania-6b, Paneth cell-enhanced expression protein, PCEE, Ania6b

    Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively).  Cyclin L2 can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). The Cyclin L2 gene is located approximately 100-200 kb from the PITSLRE/CDK11 protein kinase locus in human (1p36) and in a syngenetic location on mouse chromosome 4q.  Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

    Gene Name: CCNL2, SB138

    Gene ID
    81669, 14585859
    UniProt
    Q96S94
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