Cyclin L2 antibody (AA 309-384)

Catalog No. ABIN6655712

Product Details anti-Cyclin L2 Antibody

Target: Cyclin L2 (CCNL2)

Binding Specificity: AA 309-384

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin L2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity: Human, Mouse (Murine)

Cross-Reactivity (Details): Cross reactivity with Cyclin L2a protein from rat and chicken (called Cyclin S) is expected, as this sequence shows ~ 80 % homology with the protein from these sourcesCross reactivity with Cyclin L2a homologues from other sources has not been determined.

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using polypeptide coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This antibody is specific for human Cyclin L2a and shows negligible reactivity to Cyclin L2b or Cyclin L1 isoforms.  A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L2a protein from rat and chicken (called Cyclin S) is expected, as this sequence shows ~ 80% homology with the protein from these sources. Cross reactivity with Cyclin L2a homologues from other sources has not been determined.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 309-384 of Human Cyclin L2a protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 58 kDa in size corresponding to Cyclin L2a by western blotting in the appropriate cell lysate or extract. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation.

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:3,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

References for anti-Cyclin L2 antibody (ABIN6655712)

Kren, Unger, Abedin, Vogel, Henzler, Ahmed, Trembley: "Preclinical evaluation of cyclin dependent kinase 11 and casein kinase 2 survival kinases as RNA interference targets for triple negative breast cancer therapy." in: Breast cancer research : BCR, Vol. 17, pp. 19, (2015) (PubMed).

Loyer, Trembley, Katona, Kidd, Lahti: "Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).

Target Details for Cyclin L2

Target: Cyclin L2 (CCNL2)

Alternative Name: Cyclin L2 (CCNL2 Products)

Synonyms: CCNL2 antibody, ANIA-6B antibody, CCNM antibody, HCLA-ISO antibody, HLA-ISO antibody, PCEE antibody, SB138 antibody, CCNL1 antibody, 1700010A01Rik antibody, 1810019L15Rik antibody, 2010319M22Rik antibody, AA409936 antibody, AW261738 antibody, Ania-6b antibody, HCLA-150 antibody, Pcee antibody, cyclin L2 antibody, cyclin L2 L homeolog antibody, CCNL2 antibody, ccnl2.L antibody, Ccnl2 antibody

Background:

Synonyms: rabbit anti-Cyclin-L2 antibody, Cyclin L2, CCNL2, SB138, Paneth cell-enhanced expression protein, Cyclin Ania-6b, Paneth cell-enhanced expression protein, PCEE, Ania6b

Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively).  Cyclin L2 can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). The Cyclin L2 gene is located approximately 100-200 kb from the PITSLRE/CDK11 protein kinase locus in human (1p36) and in a syngenetic location on mouse chromosome 4q.  Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Gene Name: CCNL2, SB138

Gene ID: 81669, 14585859

UniProt: Q96S94