ATF6 antibody (N-Term)

Catalog No. ABIN6655678

Supplier Product No.: 200-301-h56

Product Details anti-ATF6 Antibody

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP)

Supplier Product No.: 200-301-h56

Supplier: Rockland

Purpose: ATF6 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-ATF6 from Grouper (Fish), Hamster, Human, Mouse, Rabbit, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-ATF6 Antibody was purified by Protein G chromatography.

Immunogen: ATF6 Antibody was produced in mice by repeated immunizations with the partial protein of human ATF6 at the n-terminus of the protein.

Clone: 70B1413-1

Isotype: IgG1 kappa

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1-5 μg/mL

Comment:

Anti-ATF6 antibody is tested for use in WB, ChIP, Flow, Flow-IC, ICC/IF, IHC-Fr, IHC-P, and IP. Expect a band approximately 90 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: ATF6 (ATF6 Products)

Background:

Synonyms: Activating transcription factor 6 alpha

Background: Anti-ATF6 antibody detects human ATF6. ATF6 is a constitutively expressed, endoplasmic reticulum (ER) membrane-anchored transcription factor. ATF6 is a key transcriptional activator of the unfolded protein response (UPR), which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER. The C-terminus of ATF6 is located in the ER lumen and its N-terminal DNA binding domain faces the cytosol. AFT6 plays a key role in the ER stress response by transmitting the ER stress signal across the ER membrane into the nucleus. The induction of new gene expression by ATF6 is an important aspect of the ER stress response. In response to certain stress conditions, ATF6 translocates from the ER to the Golgi. The 90 kDa full-length ATF6 is processed within the Golgi to its active 50 kDa form through sequential cleavage by site-1 and site-2 proteases (S1P and S2P). Proteolytic activation of ATF6 in the ER stress response is a mechanism to regulate membrane-bound factors, and is referred to as regulated intramembrane proteolysis. The N-terminal active ATF6 translocates to the nucleus where it binds to ER stress-response elements in ER stress-response genes (ERSRGs). ATF6 is a potent transcriptional activator of ERSRGs. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment. Anti-ATF6 antibody is ideal for investigators involved in apoptosis research.

Gene Name: ATF6

Gene ID: 22926

NCBI Accession: NP_031374

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response