MAPK14 antibody

Catalog No. ABIN6655670

Supplier Product No.: 600-101-hb4

Product Details anti-MAPK14 Antibody

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MAPK14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-101-hb4

Supplier: Rockland

Purpose: P38 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human p38.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-p38 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human p38 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 5 μg/mL

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-p38 Antibody has been tested in Western Blot, ELISA and Immunohistochemistry. Expect a band at ~42 kDa in western blot using appropriate lysates. Positive control whole cell lysates used NIH3T3 and PC-12 @ 1μg/mL for WB, breast cancer tissue for IHC. This antibody has been additionally validated using a p38 knock-out lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: P38 (MAPK14 Products)

Background:

Synonyms: Goat Anti-p38 Antibody, Goat Anti-MAPK14 Antibody, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, MAPK14

Background: p38, also called SAPK2A, belongs to the MAP kinase family. The MAP kinases are critical signaling proteins for many biochemical signals. They participate in transcription regulation, cellular differentiation and proliferation. Environmental signals and cytokines catalyze the kinases into bonding their substrates such as MAX and ATF2, for example, and p53 a tumor suppressing gene. p38 may be associated with diseases such as chlamydia and patellar tendinitis. Anti-p38 antibody is useful for researchers interested in signal pathways, cellular development or cancer research.

Gene Name: MAPK14

Gene ID: 1432

NCBI Accession: NP_001306

UniProt: Q16539

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins