HIST1H3A antibody (H3K27me2)

Catalog No. ABIN6655653

Product Details anti-HIST1H3A Antibody

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Binding Specificity: H3K27me2

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H3A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Purpose: Histone H3 K27me2 Antibody

Cross-Reactivity (Details): This product reacts with human Histone 3 dimethyl Lys27.

Purification: Anti-H3K27me2 Antibody was purified by affinity purification.

Sterility: Sterile filtered

Immunogen: Anti-H3K27me2 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of human histone H3, dimethylated at lysine 27.

Isotype: IgG

Application Details

Application Notes:

ChIP_Dilution: 2 μg/ChIP

ELISA_Dilution: 1:1,000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1,000

Other: Dot Blot: 1:50,000

Comment:

Anti-H3K27me2 Antibody is tested in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunofluorescence, and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 17 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HIST1H3A

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Alternative Name: HIST1H3A (HIST1H3A Products)

Synonyms: H3/A antibody, H3FA antibody, histone cluster 1 H3 family member a antibody, histone cluster 1, H3a antibody, HIST1H3A antibody, Hist1h3a antibody

Background:

Synonyms: Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/e, Histone H3/f, Histone H3/g, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called ""histone code"". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Levels of H3K27 dimethylation are higher in silent genes than in active genes suggesting that this histone modification is associated with transcriptional repression. Anti-H3K27me2 Antibody is ideal for research in Chromatin Remodeling, Epigenetics and Gene Expression.

Gene Name: HIST1H3A

Gene ID: 3020

NCBI Accession: NP_002098

UniProt: P84243