HIST1H3A antibody (H3K27me)

Catalog No. ABIN6655652

Supplier Product No.: 600-401-w54

Product Details anti-HIST1H3A Antibody

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Binding Specificity: H3K27me

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H3A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-w54

Supplier: Rockland

Purpose: Histone H3 K27me1 Antibody

Cross-Reactivity (Details): This product reacts with human Histone 3 methyl Lys27.

Purification: Anti-Histone H3 k27 me1 Antibody was purified by affinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Histone H3 K27 me1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of histone H3 containing the monomethylated lysine 27.

Isotype: IgG

Application Details

Application Notes:

ChIP_Dilution: 1 μg per IP

ELISA_Dilution: 1:500

IF_Microscopy_Dilution: 1:1,000

Western_Blot_Dilution: 1:1,000

Other: Dot Blot: 1:20,000

Comment:

Anti-Histone H3 K27 me1 Antibody is tested in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunofluorescence, and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HIST1H3A

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Alternative Name: HIST1H3A (HIST1H3A Products)

Background:

Synonyms: Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/e, Histone H3/f, Histone H3/g, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called ""histone code"". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Anti-Histone H3 K27 me1 Antibody is ideal for research in Chromatin Remodeling and Genetics.

Gene Name: HIST1H3A

Gene ID: 3020

NCBI Accession: NP_002098

UniProt: P84243