TNFAIP3 antibody (C-Term)

Catalog No. ABIN6655643

Supplier Product No.: 200-301-h52

Product Details anti-TNFAIP3 Antibody

Target: TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFAIP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Supplier Product No.: 200-301-h52

Supplier: Rockland

Purpose: A20 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-A20 from Human, Mouse and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-A20 Antibody was purified by Protein G chromatography.

Immunogen: A20 Antibody was produced in mice by repeated immunizations with recombinant human A20 protein at the c-terminus.

Clone: 59A426

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunoprecipitation_Dilution: 1-2 μg/mL

Western_Blot_Dilution: 2-4 μg/mL

Comment:

Anti-A20 antibody is tested for use in WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TNFAIP3

Target: TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))

Alternative Name: TNFAIP3 (TNFAIP3 Products)

Background:

Synonyms: OTUD7C, Tumor necrosis factor alpha-induced protein 3, TNF alpha-induced protein 3, OTU domain-containing protein 7C, A20p50, A20p37

Background: Anti-A20 antibody detects human A20. A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase. The A20 monoclonal antibody has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology. Anti-A20 antibody is ideal for investigators involved in NFkappaB and Signaling research.

Gene Name: TNFAIP3

Gene ID: 7128

NCBI Accession: NP_001257436

UniProt: P21580

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Production of Molecular Mediator of Immune Response