ZBTB24 antibody (C-Term)

Catalog No. ABIN6655627

Product Details anti-ZBTB24 Antibody

Target: ZBTB24 (Zinc Finger and BTB Domain Containing 24 (ZBTB24))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZBTB24 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Anti-Bif-1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Bif-1 from Human based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Bif-1 from other sources has not been determined.

Immunogen:

Immunogen: Bif-1 Antibody was produced in mice and was prepared by repeated immunizations at the c-terminus of the human recombinant protein Bif-1.

Immunogen Type: Peptide

Clone: 30A882-1-1

Isotype: IgG1

Application Details

Application Notes:

Application Note: Anti-Bif-1 Antibody is suitable for use in IP and WB. Expect a band approximately 23 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1-2- μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for ZBTB24

Target: ZBTB24 (Zinc Finger and BTB Domain Containing 24 (ZBTB24))

Alternative Name: Bif-1 (ZBTB24 Products)

Synonyms: BIF1 antibody, ICF2 antibody, PATZ2 antibody, ZNF450 antibody, BC055367 antibody, Bif1 antibody, Bsg1 antibody, Bif1a antibody, zfp450 antibody, zgc:194556 antibody, zinc finger and BTB domain containing 24 antibody, ZBTB24 antibody, Zbtb24 antibody, zbtb24 antibody

Background:

Synonyms: KIAA0491, Endophilin-B1, Bax-interacting factor 1, Bif-1, SH3 domain-containing GRB2-like protein B1

Background: Anti-Bif-1 Antibody detects human BIF. The Bcl-2 family consists of anti-apoptotic (or cell survival) genes, such as Bcl-2, Bcl-XL and pro-apoptotic (or cell death) genes including Bax and BAD. In addition to its anti-apoptotic effect, Bcl-2 also inhibits progression through the cell cycle. Functional interactions between family members as well as binding to other cellular proteins modulate their activities. Bcl-2 family members are important in normal tissue development, homeostasis, and disease states. Bcl-2 prevents cell death by forming heterodimers with Bax and Bad. Bax promotes cell death by forming homodimers, which can be blocked by forming heterodimers with Bcl-2 or BCL-XL. Recently, using yeast two-hybrid system Cuddeback have identified a cDNA encoding a 365 amino acid protein, which interacts physically with Bax protein and influences cell life and death. Bif-1 contains a Src homology 3 (SH3) domain in the C-terminus. It does not contain any of the conserved Bcl-2 homology (BH) domains of the Bcl-2 family proteins. Human Bif-1 protein shares 96 % and 42 % identity at the amino acids level with mouse and C. elegans, respectively. Overexpression of Bif-1 in transfected cell lines promotes Bax conformational change, caspase activation, and apoptotic cell death following growth factor withdrawal. Bif-1 RNA is abundantly expressed in heart, skeletal muscle, kidney, and placenta. Anti-BIF-1 antibody is ideal for investigators involved in cytokines and growth factor research and NFkappaB and signaling research.

Gene Name: SH3GLB1