PAPD1 antibody (C-Term)

Catalog No. ABIN6655607

Product Details anti-PAPD1 Antibody

Target: PAPD1 (MTPAP) (Mitochondrial Poly(A) Polymerase (MTPAP))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAPD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity with PAPD1 from other species not tested.

Purification: Anti-PAPD1 Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.

Immunogen:

Immunogen: Anti-PAPD1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of human PAPD1 containing an amino acid sequence from the C-terminus of the protein.

Immunogen Type: Peptide

Application Details

Application Notes:

Application Note: Anti-PAPD1 Antibody is suitable for ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 66 kDa in the appropriate cell lysate or extract.

ELISA Dilution: 1:500

Western Blot Dilution: 1:1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for PAPD1

Target: PAPD1 (MTPAP) (Mitochondrial Poly(A) Polymerase (MTPAP))

Alternative Name: PAPD1 (MTPAP Products)

Synonyms: PAPD1 antibody, SPAX4 antibody, 0610027A18Rik antibody, AW551379 antibody, Papd1 antibody, mitochondrial poly(A) polymerase antibody, MTPAP antibody, Mtpap antibody

Background:

Synonyms: Poly(A) RNA polymerase mitochondrial, PAP, PAP-associated domain-containing protein 1, Polynucleotide adenylyltransferase, Terminal uridylyltransferase 1, TUTase1, mtPAP

Background: PAPD1 is a mitochondrial RNA polymerase that generates the poly(A) tail of mitochondrial RNA's by adding AMP monomers to the 3'-end. PAPD1 might also be responsible for the introduction of UAA stop codons, which are not encoded by the mitochondrial DNA, in some RNA molecules. Anti-PAPD1 Antibody is ideal for research in Gene Expression, Transcription and Translation.

Gene Name: MTPAP

Gene ID: 55149

NCBI Accession: NP_060579

UniProt: Q9NVV4