CD4 antibody (APC)

Catalog No. ABIN6655530

Supplier Product No.: 200-326-n18

Product Details anti-CD4 Antibody (APC)

Target: CD4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 200-326-n18

Supplier: Rockland

Purpose: CD4 Allophycocyanin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD4, Cynomolgus, Rhesus, and chimpanzee.

Purification: Allophycocyanin conjugated CD4 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD4.

Sterility: Sterile filtered

Immunogen: Anti-CD4 Antibody (Monoclonal) was produced by repeated immunizations with CD4 antigen.

Clone: OKT4

Isotype: IgG2b kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5μL/test/1x10e5 to 1x10e8 cells

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: User Optimized

Comment:

Anti-CD4 is tested in Flow Cytometry and useful for Immunoprecipitation, Immunofluorescence, Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD4

Target: CD4

Alternative Name: CD4 (CD4 Products)

Background:

Synonyms: T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, FITC anti-human CD4, RPA-T4, APC, T4

Background: The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The OKT4 antibody is widely used as a phenotypic marker for CD4 expression.

Gene Name: CD4

Gene ID: 920

NCBI Accession: NP_000607

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events