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IKK alpha antibody

Rockland 200-301-h82 CHUK Reactivity: Human WB, IHC, IF, IP, FACS, ChIP Host: Mouse Monoclonal 14A231 unconjugated
Rockland
Catalog No. ABIN6655526
Supplier Product No.: 200-301-h82
  • Target See all IKK alpha (CHUK) Antibodies
    IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
    Reactivity
    • 120
    • 70
    • 54
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    Human
    Host
    • 130
    • 13
    • 1
    Mouse
    Clonality
    • 121
    • 23
    Monoclonal
    Conjugate
    • 78
    • 12
    • 6
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This IKK alpha antibody is un-conjugated
    Application
    • 110
    • 57
    • 35
    • 27
    • 26
    • 17
    • 15
    • 14
    • 10
    • 6
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP)
    Supplier Product No.
    200-301-h82
    Supplier
    Rockland
    Purpose
    IKK alpha Antibody
    Cross-Reactivity (Details)
    A BLAST analysis was used to suggest cross-reactivity with IKKα from Human, Mouse and New World Monkey based on 100 % homology with the immunizing sequence.
    Purification
    Anti-IKKα Antibody was purified by Protein G chromatography.
    Immunogen
    IKK alpha Antibody was produced in mice prepared by repeated immunizations by a His-tagged human IKK alpha protein at full-length.
    Clone
    14A231
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CHUK Primary Antibody
  • Application Notes

    Immunoprecipitation_Dilution: 1-2 μg/mL

    Immunohistochemistry_Dilution: 5 μg/mL

    Flow_Cytometry_Dilution: 0.25-0.5 μg/10^6 cells

    Western_Blot_Dilution: 1 μg/mL

    Comment

    Anti-IKK alpha antibody is tested for use in WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, and IP. Expect a band approximately 85-90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.05 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
    Alternative Name
    IKK alpha (CHUK Products)
    Background

    Synonyms: IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, 2.7.11.10, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA, Transcription factor 16, TCF-16

    Background: Anti-IKK alpha antibody detects human IKK alpha. NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK alpha, IKK beta, and IKK gamma. In vitro, IKK alpha and IKK beta can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK alpha and IKK beta are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK alpha gene in mice results in skin and limb abnormalities and death of newborns. Anti-IKK alpha antibody is ideal for investigators involved in cytokines and growth factor and NFkappaB research.

    Gene Name: CHUK

    Gene ID
    1147
    NCBI Accession
    NP_001269
    UniProt
    O15111
    Pathways
    PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins
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