ErbB2/Her2 antibody (Extracellular Domain)

Catalog No. ABIN6655455

Supplier Product No.: 900-c01-b37

Product Details anti-ErbB2/Her2 Antibody

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 900-c01-b37

Supplier: Rockland

Purpose: c-erbB2 Antibody

Cross-Reactivity (Details): The antibody is specific for human c-erbB2 protein.

Purification: Anti-erbB2 Antibody is clarified tissue culture supernate.

Sterility: Sterile filtered

Immunogen: erbB2 monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues encoding the extracellular domain of human c-erbB2 protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.

Clone: SP3

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:200-1:500

Comment:

Anti-erbB2 Antibody monoclonal antibody contains TBS/1% BSA. Anti-erbB2 Antibody is tested with IHC and is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 185 kDa in size corresponding to c-erbB2 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. C-erbB2 is found in human breast carcinoma tissue at the cell membrane.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: See application note.

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date.

Expiry Date: 12 months

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: c-erbB2 (ErbB2/Her2 Products)

Background:

Synonyms: rabbit anti-c-erbB2 Antibody, Receptor tyrosine-protein kinase erbB-2, p185erbB2, C-erbB-2, NEU proto-oncogene, tyrosine kinase-type cell surface receptor HER2, MLN 19, CD340

Background: The ERBB2 gene encodes a member of the tyrosine protein kinase superfamily that is an essential component of a neuregulin-receptor complex called c-erbB2 (also known as Receptor tyrosine-protein kinase erbB-2, p185erbB2, C-erbB-2, NEU proto-oncogene, tyrosine kinase-type cell surface receptor HER2, MLN 19 and CD340), although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. C-erbB2 is not activated by EGF, TGF-alpha and amphiregulin. Defects in ERBB2 are associated with gastric cancer, familial glioma of brain (glioblastoma multiforme), ovarian cancer and lung cancer (adenocarcinoma of lung). c-erbB2 antibody is ideal for Cancer and Signal Transduction research.

Gene Name: ERBB2

Gene ID: 2064

NCBI Accession: NP_001005862

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development