CD19 antibody (APC)

Catalog No. ABIN6655428

Supplier Product No.: 200-326-n24

Product Details anti-CD19 Antibody (APC)

Target: CD19 (CD19 Molecule (CD19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Supplier Product No.: 200-326-n24

Supplier: Rockland

Purpose: CD19 Allophycocyanin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD19.

Purification: Allophycocyanin conjugated CD19 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD19.

Sterility: Sterile filtered

Immunogen: Anti-CD19 Antibody (Monoclonal) was produced by repeated immunizations with CD19 antigen.

Clone: HIB19

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5μL/test/1x10e5 to 1x10e8 cells

Comment:

Anti-CD19 is tested for Flow Cytometry and useful in Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: None

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19 (CD19 Products)

Background:

Synonyms: B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19

Background: The HIB19 antibody reacts with human CD19, 95 kDa glycoprotein which acts as a co-receptor, along with CD21, CD81 and CD225, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.The HIB19 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

Gene Name: CD19

Gene ID: 930

NCBI Accession: NP_001171569

UniProt: P15391

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway