TLR7 antibody

Catalog No. ABIN6655379

Supplier Product No.: 200-301-i29

Product Details anti-TLR7 Antibody

Target: TLR7 (Toll-Like Receptor 7 (TLR7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Supplier Product No.: 200-301-i29

Supplier: Rockland

Purpose: TLR7 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR7 from human based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR7 Antibody was purified by Protein G chromatography.

Immunogen: TLR7 Antibody was produced in mice prepared by repeated immunizations with an internal portion of human TLR7 recombinant protein.

Clone: IMG4G6

Isotype: IgG1 kappa

Application Details

Application Notes:

Flow_Cytometry_Dilution: 1-2 μg/10^6 cells

Western_Blot_Dilution: 10 μg/mL

Comment:

Anti-TLR7 Antibody is tested for use in WB, Flow, Flow-IC, ICC/IF, and IHC. Expect a band approximately 121kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TLR7

Target: TLR7 (Toll-Like Receptor 7 (TLR7))

Alternative Name: TLR7 (TLR7 Products)

Background:

Synonyms: Toll-like receptor 7

Background: Anti-TLR7 Antibody detects human TLR7. TLR7 is a 1049 amino acid long highly conserved membrane protein belonging to the Toll-like receptor (TLR) family with 27 LRR (leucine-rich) repeats and a TIR domain. It plays a fundamental role in pathogen recognition and activation of innate immunity. TLR7 is activated by infections with single-stranded RNA viruses, including influenza virus and vesicular stomatitis virus (VSV). It is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8. It acts via MyD88 and TRAF6, leading to NF-kappaB activation, cytokine secretion and the inflammatory response. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Anti-TLR7 Antibody is ideal for investigators that are involved in cytokin and grow factor research.

Gene Name: TLR7

Gene ID: 51284

NCBI Accession: NP_057646

UniProt: Q9NYK1

Pathways: TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades