TRF2 antibody

Catalog No. ABIN6655376

Product Details anti-TRF2 Antibody

Target: TRF2 (TERF2) (Telomeric Repeat Binding Factor 2 (TERF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Purpose: TRF2 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TRF2 from Human, Mouse, Muntjac (deer), and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-TRF2 Antibody was purified by Protein G chromatography.

Immunogen: TRF2 Antibody was produced in mice by repeated immunizations with a full-length TRF2 human protein.

Clone: 4A794-15

Isotype: IgG1 kappa

Application Details

Application Notes:

ChIP_Dilution: User Optimized

Immunoprecipitation_Dilution: 2 μg/10^6 cells

ELISA_Dilution: 2 μg/mL

Immunohistochemistry_Dilution: 5 μg/mL

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 2-4 μg/mL

Comment:

Anti-TRF2 Antibody is tested for use in WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, and IP. Expect a band approximately 55.6 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TRF2

Target: TRF2 (TERF2) (Telomeric Repeat Binding Factor 2 (TERF2))

Alternative Name: TERF2 (TERF2 Products)

Synonyms: TERF2 antibody, TRBF2 antibody, TRF2 antibody, trf2 antibody, telomeric repeat binding factor 2 antibody, telomeric repeat binding factor 2 L homeolog antibody, TERF2 antibody, Terf2 antibody, terf2.L antibody

Background:

Synonyms: TRBF2, TRF2, Telomeric repeat-binding factor 2, TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein

Background: TRF2 antibody detects human, mouse, deer and rat TRF2. TRF2 is an ubiquitously expressed protein that is implicated in the control of telomere length. TRF2, like TRF1, contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types. Anti TRF2 antibody is ideal for investigators involved in apoptosis and chromatin research.

Gene Name: TERF2

Gene ID: 7014

NCBI Accession: NP_005643

UniProt: Q15554

Pathways: Cell Division Cycle, Telomere Maintenance