CTSA antibody

Catalog No. ABIN6655368

Supplier Product No.: 100-401-395

Product Details anti-CTSA Antibody

Target: CTSA (Cathepsin A (CTSA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTSA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 100-401-395

Supplier: Rockland

Purpose: Protective Protein/Cathepsin A Antibody

Cross-Reactivity (Details): Based on sequence we expect this antibody to react with PPCA from other sources, although specific reactivity has not been confirmed.

Purification: This antiserum is directed against PPCA and reacts with the PPCA from human tissues.

Sterility: Sterile filtered

Immunogen: This whole rabbit serum was prepared by repeated immunizations with native 54-kDa human Protective Protein/Cathepsin A (PPCA) precursor, purified from the medium of baculovirus infected insect cells.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:1,000 - 1:5,000

Immunohistochemistry_Dilution: 1:500

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Comment:

Suggested Applications: IP
Anti-PPCA has been tested by immunohistochemistry and is suitable for the detection of human PPCA (54-, 32-, and 20-kDa) on Western blot, by immunoprecipitation, immunocytochemistry, and ELISA of either transfected cells (e.g. COS-1 cells) or primary cells (e.g. fibroblasts).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CTSA

Target: CTSA (Cathepsin A (CTSA))

Alternative Name: Protective Protein/Cathepsin A (CTSA Products)

Background:

Synonyms: rabbit anti-Protective Protein Antibody, rabbit anti-Cathepsin A Antibody, Beta Galactosidase Protective Protein antibody, Carboxypeptidase C antibody, Cathepsin A antibody, CTSA antibody, Glactosialidosis antibody, GLB2 antibody, Goldberg Syndrome antibody

Background: Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54- kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20- kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH . Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Gene Name: CTSA, PPGB

Gene ID: 5476

UniProt: P10619