TLR3 antibody

Catalog No. ABIN6655315

Supplier Product No.: 200-301-i24

Product Details anti-TLR3 Antibody

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

Supplier Product No.: 200-301-i24

Supplier: Rockland

Purpose: TLR3 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR3 from dog, human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR3 Antibody was purified by Protein G chromatography.

Immunogen: TLR3 Antibody was produced in mice prepared by repeated immunizations with a peptide corresponding to internal amino acid sequences in the human protein TLR3.

Clone: 40C1285-6

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunoprecipitation_Dilution: 2 μg/10^6 cells

Immunohistochemistry_Dilution: 10 μg/mL

Flow_Cytometry_Dilution: 2-4 μg/10^6 cells

Western_Blot_Dilution: 1-3 μg/mL

Comment:

Anti-TLR3 Antibody is tested for use in WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP, and Bioactivity. Expect a band approximately 120kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TLR3

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Alternative Name: TLR3 (TLR3 Products)

Background:

Synonyms: Toll-like receptor 3, CD283

Background: Anti-TLR3 Antibody detects human TLR3. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. TLR3 cDNA codes for a protein with approximate molecular weight of 120 kDa. TLR3 has a restricted expression pattern being expressed in dendritic cells (DC). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes . The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting. Anti-TLR3 Antibody is ideal for investigators involved in cytokines and growth factor research.

Gene Name: TLR3

Gene ID: 7098

NCBI Accession: NP_003256

UniProt: O15455

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades