TLR3 antibody
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- Target See all TLR3 Antibodies
- TLR3 (Toll-Like Receptor 3 (TLR3))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TLR3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
- Purification
- Anti-TLR3 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TLR3 from dog, human and mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TLR3 from other sources has not been determined.
- Immunogen
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Immunogen: TLR3 Antibody was produced in mice prepared by repeated immunizations with a peptide corresponding to internal amino acid sequences in the human protein TLR3.
Immunogen Type: Peptide
- Clone
- 40C1285-6
- Isotype
- IgG1
- Top Product
- Discover our top product TLR3 Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 10 μg/mL
Application Note: Anti-TLR3 Antibody is suitable for use in Flow (CS), Flow (I), IF/ICC, IHC (p), IP and WB. Expect a band approximately 120 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Immunoprecipitation Dilution: 2 μg/10^6 cells
Flow Cytometry Dilution: 2-4 μg/10^6 cells
Western Blot Dilution: 1-3 μg/mL
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
0.05 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- TLR3 (Toll-Like Receptor 3 (TLR3))
- Alternative Name
- TLR3 (TLR3 Products)
- Synonyms
- CD283 antibody, IIAE2 antibody, AI957183 antibody, zgc:123006 antibody, toll like receptor 3 antibody, toll-like receptor 3 antibody, TLR3 antibody, Tlr3 antibody, tlr3 antibody
- Background
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Synonyms: Toll-like receptor 3, CD283
Background: Anti-TLR3 Antibody detects human TLR3. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. TLR3 cDNA codes for a protein with approximate molecular weight of 120 kDa. TLR3 has a restricted expression pattern being expressed in dendritic cells (DC). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes . The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting. Anti-TLR3 Antibody is ideal for investigators involved in cytokines and growth factor research.
Gene Name: TLR3
- Gene ID
- 7098
- NCBI Accession
- NP_003256
- UniProt
- O15455
- Pathways
- TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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