BAX antibody (Internal Region)

Catalog No. ABIN6655295

Supplier Product No.: 200-c01-b34

Product Details anti-BAX Antibody

Target: BAX (BCL2-Associated X Protein (BAX))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Supplier Product No.: 200-c01-b34

Supplier: Rockland

Purpose: BAX Antibody

Cross-Reactivity (Details): This antibody is specific for human BAX protein.

Purification: This product was purified from concentrated tissue culture supernate by Protein A/G chromatography.

Sterility: Sterile filtered

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human BAX protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.

Clone: SP47

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:50

Flow_Cytometry_Dilution: 1:100

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Comment:

This monoclonal antibody is tested for IHC and suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to BAX protein by western blotting in the appropriate cell lysate or extract. BAX is found in normal breast tissue as a cytoplasmic protein and also at the cell membrane. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. Contains 1% BSA.

Expiry Date: 12 months

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: BAX (BAX Products)

Background:

Synonyms: BAX Bcl-2-like protein 4 BCL2L4

Background: BAX, also known as Bcl-2-like protein 4 and BCL2L4 is known to accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. BAX induces the release of cytochrome c, activation of CASP3, and thereby apoptosis. BAX protein is expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histyocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Isoform alpha is considered to be the 'canonical' sequence.

Gene Name: BAX

Gene ID: 581

NCBI Accession: NP_001278357

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response