MECP2 antibody (C-Term)

Catalog No. ABIN6655271

Supplier Product No.: 600-401-x39

Product Details anti-MECP2 Antibody

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Supplier Product No.: 600-401-x39

Supplier: Rockland

Purpose: MeCP2 Antibody

Cross-Reactivity (Details): This product reacts with human MeCP2.

Purification: Anti-MeCP2 Antibody was purified by affinity purification.

Sterility: Sterile filtered

Immunogen: Anti-MeCP2 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing the C-terminal region of human MeCP2.

Isotype: IgG

Application Details

Application Notes:

ChIP_Dilution: 5 μg per IP

ELISA_Dilution: 1:1,000

Western_Blot_Dilution: 1:1,000

Comment:

Anti-MeCP2 Antibody is tested for Chromatin Immunoprecipitation, ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 70 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MeCP2 (MECP2 Products)

Background:

Synonyms: Methyl-CpG-binding protein 2, MeCP-2 protein, AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT

Background: MeCP2 is a chromosomal protein with abundant binding sites in the chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism. Anti-MeCP2 Antibody is ideal for research in Epigenetics, Embryonic Development and Neuroscience.

Gene Name: MECP2

Gene ID: 4204

NCBI Accession: NP_001104262

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane