Progesterone Receptor antibody (AA 412-526)

Catalog No. ABIN6655214

Supplier Product No.: 900-c01-b36

Product Details anti-Progesterone Receptor Antibody

Target: Progesterone Receptor (PGR)

Binding Specificity: AA 412-526

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Progesterone Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 900-c01-b36

Supplier: Rockland

Purpose: Progesterone Receptor Antibody

Cross-Reactivity (Details): The antibody is specific for human Progesterone Receptor protein.

Purification: This product is clarified tissue culture supernate.

Sterility: Sterile filtered

Immunogen: Anti-Progesterone Receptor monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 412-526 of human Progesterone Receptor protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.

Clone: SP2

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:400

Western_Blot_Dilution: 1:200-1:500

Comment:

This monoclonal antibody contains TBS/1% BSA. Anti-Progesterone Receptor has been tested in IHC and is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 116 kDa in size corresponding to PgR isoform B (the 'canonical' sequence) protein by western blotting in the appropriate cell lysate or extract. Progesterone Receptor is found in human Breast ductal carcinoma and is a nuclear protein. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: See application note.

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date.

Expiry Date: 6 months

References for anti-Progesterone Receptor antibody (ABIN6655214)

Huang, Zhu, Szekeres, Xia: "Development of new rabbit monoclonal antibody to estrogen receptor: immunohistochemical assessment on formalin-fixed, paraffin-embedded tissue sections." in: Applied immunohistochemistry & molecular morphology : AIMM, Vol. 13, Issue 1, pp. 91-5, (2005) (PubMed).

Cano, Milanezi, Leitão, Ricardo, Brito, Schmitt: "Estimation of hormone receptor status in fine-needle aspirates and paraffin-embedded sections from breast cancer using the novel rabbit monoclonal antibodies SP1 and SP2." in: Diagnostic cytopathology, Vol. 29, Issue 4, pp. 207-11, (2004) (PubMed).

Target Details for Progesterone Receptor

Target: Progesterone Receptor (PGR)

Alternative Name: Progesterone Receptor (PGR Products)

Background:

Synonyms: rabbit anti-Progesterone Receptor Antibody, PgR, PR, nuclear receptor subfamily 3 group C member 3

Background: The PGR gene encodes a member of the steroid receptor superfamily referred to as progesterone receptor (also known as PgR, PR and nuclear receptor subfamily 3 group C member 3). Progesterone receptor mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform A only, and mediate their own response genes and physiologic effects with little overlap. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.

Gene Name: PGR

Gene ID: 5241

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration