MBD1 antibody (N-Term)

Catalog No. ABIN6655183

Product Details anti-MBD1 Antibody

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Cross-Reactivity (Details): Cross reactivity with other species was not tested.

Purification: Anti-MBD1 Antibody was purified by affinity purification.

Immunogen:

Immunogen: Anti-MBD1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the N-terminus of human MBD1.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-MBD1 Antibody is suitable for Chromatin Immunoprecipitation, ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75 kDa in the appropriate cell lysate or extract.

ChIP Dilution: 1.5 μg per IP

ELISA Dilution: 1:1,000

Western Blot Dilution: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300/p>

Stabilizer: None

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Alternative Name: MBD1 (MBD1 Products)

Synonyms: CXXC3 antibody, PCM1 antibody, RFT antibody, Cxxc3 antibody, CCDC11 antibody, methyl-CpG binding domain protein 1 antibody, MBD1 antibody, Mbd1 antibody

Background:

Synonyms: Methyl-CpG-binding domain protein 1, CXXC-type zinc finger protein 3, Methyl-CpG-binding protein MBD1, Protein containing methyl-CpG-binding domain 1, CXXC3, PCM1

Background: MBD1 is a transcriptional repressor that specifically binds to methylated CpG dinucleotides in promoter sequences. MBD1 acts by recruiting a variety of histone deacetylases (HDAC's) and chromatin remodelling factors. MBD1-dependent transcriptional repression is mediated by ATF7IP through the recruitment of factors such as the histone methyltransferase SETDB1. MBD1 probably forms a complex with SETDB1 and ATF7IP which couples DNA methylation to H3K9 trimethylation and represses transcription. Anti-MBD1 Antibody is ideal for research in Epigenetics, Gene Expression and Chromatin Remodeling.

Gene Name: MBD1

Gene ID: 4152

NCBI Accession: NP_001191065

UniProt: Q9UIS9