CD25 antibody (FITC)

Catalog No. ABIN6655112

Supplier Product No.: 200-302-n25

Product Details anti-CD25 Antibody (FITC)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Supplier Product No.: 200-302-n25

Supplier: Rockland

Purpose: CD25 Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD25, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Pigtailed Macaque.

Purification: Fluorescein conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD25.

Sterility: Sterile filtered

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with CD25 antigen.

Clone: BC96

Isotype: IgG1 kappa

Labeling Ratio: 4-6

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Comment:

Anti-CD25 is tested for Flow Cytometry and is useful for Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

References for anti-CD25 antibody (FITC) (ABIN6655112)

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, (1991) (PubMed).

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 (IL2RA Products)

Background:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55

Background: CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Gene Name: IL2RA

Gene ID: 3559

NCBI Accession: NP_000408

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation