Cyclin L1 antibody (AA 314-369)

Catalog No. ABIN6655105

Product Details anti-Cyclin L1 Antibody

Target: Cyclin L1 (CCNL1)

Binding Specificity: AA 314-369

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity with Cyclin L1a protein from dog, rat, mouse and chicken is expected, as this sequence shows 87-96 % homology with the protein from these sourcesCross reactivity with Cyclin L1a homologues from other sources has not been determined.

Purification: The product was purified from monospecific antiserum by Protein A chromatography. This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1b or Cyclin L2 isoforms.  A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L1a protein from dog, rat, mouse and chicken is expected, as this sequence shows 87-96% homology with the protein from these sources. Cross reactivity with Cyclin L1a homologues from other sources has not been determined.

Immunogen:

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 314-369 of Human Cyclin L1a protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 10 mg/mL - 20 μg/mL

Application Note: This Protein A purified antibody has been tested for use in ELISA, immunohistochemistry, western blot, and immunoprecipitation.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 59 kDa in size corresponding to Cyclin L1a by western blotting in the appropriate cell lysate or extract.

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:25,000

Western Blot Dilution: 1:2,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

References for anti-Cyclin L1 antibody (ABIN6655105)

Loyer, Trembley, Katona, Kidd, Lahti: "Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).

Target Details for Cyclin L1

Target: Cyclin L1 (CCNL1)

Alternative Name: Cyclin L1 (CCNL1 Products)

Synonyms: ANIA6A antibody, PRO1073 antibody, ania-6a antibody, 2610030E23Rik antibody, AU018493 antibody, Ccnl antibody, Ania-6a antibody, ccnl1 antibody, fd99b09 antibody, wu:fd99b09 antibody, zgc:55544 antibody, MCM2 antibody, cyclin-L1 antibody, MGC132032 antibody, bm-001 antibody, pro1073 antibody, MGC108436 antibody, Ccnl1 antibody, cyclin L1 antibody, cyclin L1b antibody, cyclin-L1 antibody, cyclin L1 L homeolog antibody, cyclin L1 S homeolog antibody, CCNL1 antibody, Ccnl1 antibody, ccnl1b antibody, LOC485730 antibody, ccnl1.L antibody, ccnl1 antibody, LOC100136245 antibody, ccnl1.S antibody

Background:

Synonyms: rabbit anti-Cyclin L1 antibody, Cyclin-L, Cyclin L beta, Cyclin L gamma, CCNL1

Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Gene Name: CCNL1

Gene ID: 57018

NCBI Accession: NP_064703

UniProt: Q9UK58