Calbindin-D antibody
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- Target See all Calbindin-D products
- Calbindin-D
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Calbindin-D antibody is un-conjugated
- Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Cross-Reactivity (Details)
- Cross reactivity with Calbindin-D from other sources has not been determined.
- Purification
- Anti-Calbindin-D antibody was purified from mouse ascites by protein A chromatography. This product reacts with human, mouse, bovine, and rat.
- Immunogen
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Immunogen: Calbindin-D antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to bovine kidney calbindin-D.
Immunogen Type: Recombinant Protein
- Clone
- CB-D7
- Isotype
- IgG1
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- Application Notes
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Immunohistochemistry Dilution: 1:100-1:500
Application Note: Anti-Calbindin-D is useful for Immunohistochemistry -P and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~30 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:10,000
Western Blot Dilution: 0.5-1 μg/mL
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Neutral PBS
- Buffer
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Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- Calbindin-D
- Abstract
- Calbindin-D Products
- Background
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Synonyms: CALB, D-28K, CALBINDIN, 28-KD, Calbindin D28, Vitamin D-dependent calcium-binding protein, avian-type
Background: The human calbindin D28k (CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al. (1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization. This antibody is suitable for researchers interested in transcription factors, nuclear receptors, cancer research, and nutritional and metobolic diseases.
Gene Name: CALB1
- Gene ID
- 793
- NCBI Accession
- NP_004920
- UniProt
- P05937
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