Calretinin antibody

Catalog No. ABIN6655054

Product Details anti-Calretinin Antibody

Target: Calretinin (CALB2) (Calbindin 2 (CALB2))

Reactivity: Guinea Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This Calretinin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Cross-Reactivity: Guinea Pig, Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with Calretinin from other species has not been determined

Purification: Anti-Calretinin Antibody is directed against guinea pig Calretinin protein. The antibody was purified from monospecific antiserum by Protein A chromatography. Anti-Calretinin antibody reacts with Calretinin from rat and guinea pig sources. . However, reactivity is also expected from the following species based on 100% sequence homology: human, mouse, non-human primates.

Immunogen:

Immunogen: Anti-Calretinin Antibody was produced by repeated immunizations with native guinea pig calretinin.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-Calretinin antibody is suitable for use in ELISA, Western Blotting, IHC and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to the calretinin protein in Western blots using rat brain lysate. Use this calretinin antibody for Immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M Tris Cl, 0.15 M Sodium Chloride, 0.001 M EDTA, pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

References for anti-Calretinin antibody (ABIN6655054)

Molgaard, Ulrichsen, Boggild, Holm, Vaegter, Nyengaard, Glerup: "Immunofluorescent visualization of mouse interneuron subtypes." in: F1000Research, Vol. 3, pp. 242, (2014) (PubMed).

Target Details for Calretinin

Target: Calretinin (CALB2) (Calbindin 2 (CALB2))

Alternative Name: Calretinin (CALB2 Products)

Synonyms: CAB29 antibody, CAL2 antibody, CR antibody, calb2l antibody, wu:fq18e08 antibody, zgc:73115 antibody, calb2 antibody, wu:fq17g09 antibody, zgc:73099 antibody, calbindin 2 antibody, calbindin 2a antibody, calbindin 2b antibody, CALB2 antibody, Calb2 antibody, calb2a antibody, calb2b antibody

Background:

Synonyms: CR, Calb2, CAB29

Background: Calretinin antibody reacts with calretinin which is a calcium-binding protein involved in calcium signaling. Calretinin is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium. Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas. Anti-Calretinin antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CALB2

Gene ID: 117059

NCBI Accession: NP_446440

UniProt: P47728