Calretinin antibody

Catalog No. ABIN6655054

Supplier Product No.: 200-601-d13

Product Details anti-Calretinin Antibody

Target: Calretinin (CALB2) (Calbindin 2 (CALB2))

Reactivity: Guinea Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This Calretinin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Supplier Product No.: 200-601-d13

Supplier: Rockland

Purpose: Calretinin Antibody

Cross-Reactivity (Details): Anti-Calretinin Antibody is directed against guinea pig Calretinin protein.

Purification: The antibody was purified from monospecific antiserum by Protein A chromatography.

Immunogen: Anti-Calretinin Antibody was produced by repeated immunizations with native guinea pig calretinin.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:500

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Comment:

Suggested Applications: IF
Anti-Calretinin antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to the calretinin protein in Western blots using rat brain lysate. Use this calretinin antibody for Immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.01 M Tris Cl, 0.15 M Sodium Chloride, 0.001 M EDTA, pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Calretinin antibody (ABIN6655054)

Molgaard, Ulrichsen, Boggild, Holm, Vaegter, Nyengaard, Glerup: "Immunofluorescent visualization of mouse interneuron subtypes." in: F1000Research, Vol. 3, pp. 242, (2014) (PubMed).

Target Details for Calretinin

Target: Calretinin (CALB2) (Calbindin 2 (CALB2))

Alternative Name: Calretinin (CALB2 Products)

Background:

Synonyms: CR, Calb2, CAB29

Background: Calretinin antibody reacts with calretinin which is a calcium-binding protein involved in calcium signaling. Calretinin is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium. Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas. Anti-Calretinin antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CALB2

Gene ID: 117059

NCBI Accession: NP_446440

UniProt: P47728