GFAP antibody

Catalog No. ABIN6655053

Supplier Product No.: 200-901-d60

Product Details anti-GFAP Antibody

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Cow

Host: Chicken

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 200-901-d60

Supplier: Rockland

Purpose: Glial Fibrillary Acidic Protein (GFAP) Antibody

Cross-Reactivity (Details): Anti-GFAP Antibody is directed against bovine GFAP.

Purification: The antibody is a total IgY fraction.

Immunogen: Anti-GFAP Antibody was produced by repeated immunizations with recombinant and extensively purified bovine GFAP.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:1000

Western_Blot_Dilution: 1:10000

Comment:

Suggested Applications: IHC
Anti-Glial Fibrillary Acidic Protein (Chicken) antibody is tested for use in Western Blotting, ICC, and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to the GFAP proteins in the appropriate cell lysate or extract. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFAP antibody (ABIN6655053)

Gleichman, Kawaguchi, Sofroniew, Carmichael: "A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences." in: Nature communications, Vol. 14, Issue 1, pp. 7426, (2023) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Abstract: GFAP Products

Background:

Synonyms: gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, cb345. Glial fibrillary acidic protein, GFAP antibody, neuroscience antibodies

Background: GFAP Antibody detects GFAP. Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers. Therefore, GFAP antibody is ideal for investigators involved in neuropathologic diseases and more generally in Neuroscience.

Gene Name: GFAP

Gene ID: 281189

UniProt: Q28115