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KDELR1 antibody

Rockland 500-301-f86 KDELR1 Reactivity: Cow WB, IF, IHC, IP Host: Mouse Monoclonal KR-10 unconjugated
Rockland
Catalog No. ABIN6655048
Supplier Product No.: 500-301-f86
  • Target See all KDELR1 Antibodies
    KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))
    Reactivity
    • 16
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cow
    Host
    • 15
    • 3
    Mouse
    Clonality
    • 13
    • 5
    Monoclonal
    Conjugate
    • 13
    • 1
    • 1
    • 1
    • 1
    • 1
    This KDELR1 antibody is un-conjugated
    Application
    • 18
    • 8
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Supplier Product No.
    500-301-f86
    Supplier
    Rockland
    Purpose
    KDEL Receptor Antibody
    Cross-Reactivity (Details)
    A BLAST analysis was used to suggest cross-reactivity with KDEL from Human, Monkey, Rat, Mouse, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, and Xenopus based on 100 % homology with the immunizing sequence.
    Purification
    Anti-KDEL Antibody is clarified mouse ascites fluid.
    Sterility
    Sterile filtered
    Immunogen
    KDEL Receptor Antibody was produced in mice by repeated immunizations raised against a 21 residue synthetic peptide based on the bovine KDEL receptor and the synthetic peptide coupled to KLH.
    Clone
    KR-10
    Isotype
    IgG1
    Top Product
    Discover our top product KDELR1 Primary Antibody
  • Application Notes

    Western_Blot_Dilution: 1:1000

    Other: ICC/IF: 1:1000

    Comment

    Anti-KDEL Antibody is tested for WB and IF microscopy and suitable for IP and IHC. Expect a band approximately ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: 0.09 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))
    Alternative Name
    KDELR1 (KDELR1 Products)
    Background

    Synonyms: mouse anti-KDEL Receptor Antibody, ERD2, ERD2.1, ERD21, HDEL, KDEL, KDEL R1, KDELR1, PM23, Lys Asp Glu Leu, K-D-E-L, ER lumen protein retaining receptor 1, KDEL endoplasmic reticulum protein retention, Kdelr1

    Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention. There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity, and all are located within the cis-Golgi and its intermediate compartments. In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding. It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal.

    Gene Name: Kdelr1

    Gene ID
    618184
    NCBI Accession
    NP_001069963
    UniProt
    P33946
    Pathways
    Maintenance of Protein Location
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