KDELR1 antibody
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- Target See all KDELR1 Antibodies
- KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))
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Reactivity
- Cow
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This KDELR1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Supplier Product No.
- 500-301-f86
- Supplier
- Rockland
- Purpose
- KDEL Receptor Antibody
- Cross-Reactivity (Details)
- A BLAST analysis was used to suggest cross-reactivity with KDEL from Human, Monkey, Rat, Mouse, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, and Xenopus based on 100 % homology with the immunizing sequence.
- Purification
- Anti-KDEL Antibody is clarified mouse ascites fluid.
- Sterility
- Sterile filtered
- Immunogen
- KDEL Receptor Antibody was produced in mice by repeated immunizations raised against a 21 residue synthetic peptide based on the bovine KDEL receptor and the synthetic peptide coupled to KLH.
- Clone
- KR-10
- Isotype
- IgG1
- Top Product
- Discover our top product KDELR1 Primary Antibody
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- Application Notes
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Western_Blot_Dilution: 1:1000
Other: ICC/IF: 1:1000
- Comment
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Anti-KDEL Antibody is tested for WB and IF microscopy and suitable for IP and IHC. Expect a band approximately ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.09 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))
- Alternative Name
- KDELR1 (KDELR1 Products)
- Background
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Synonyms: mouse anti-KDEL Receptor Antibody, ERD2, ERD2.1, ERD21, HDEL, KDEL, KDEL R1, KDELR1, PM23, Lys Asp Glu Leu, K-D-E-L, ER lumen protein retaining receptor 1, KDEL endoplasmic reticulum protein retention, Kdelr1
Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention. There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity, and all are located within the cis-Golgi and its intermediate compartments. In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding. It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal.
Gene Name: Kdelr1
- Gene ID
- 618184
- NCBI Accession
- NP_001069963
- UniProt
- P33946
- Pathways
- Maintenance of Protein Location
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