Ubiquitin B antibody

Catalog No. ABIN6655045

Supplier Product No.: 200-301-g61

Product Details anti-Ubiquitin B Antibody

Target: Ubiquitin B (UBB)

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Supplier Product No.: 200-301-g61

Supplier: Rockland

Purpose: Ubiquitin Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with ubiquitin from Human, mouse, rat, and bovine based on 100 % homology with the immunizing sequence.

Purification: Anti-Ubiquitin Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Ubiquitin Antibody was produced in mice by repeated immunizations raised against native bovine ubiquitin conjugated to KLH.

Clone: 5B9-B3

Isotype: IgG2a kappa

Application Details

Application Notes:

ELISA_Dilution: 1:200

Western_Blot_Dilution: 1:1000

Comment:

Anti-Ubiquitin Antibody is tested for use in WB and IF. Expect a band approximately ~10kDa corresponding to free ubiquitin. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Ubiquitin B

Target: Ubiquitin B (UBB)

Alternative Name: UBB (UBB Products)

Background:

Synonyms: Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B

Background: Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity. The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate. Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkBα and p27.

Gene Name: UBB

Gene ID: 281370

NCBI Accession: NP_776558

UniProt: P0CG53

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway