Anthrax Protective Antigen antibody (Internal Region)
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- Target See all Anthrax Protective Antigen (PA) products
- Anthrax Protective Antigen (PA)
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Binding Specificity
- Internal Region
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Reactivity
- Bacillus anthracis
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Anthrax Protective Antigen antibody is un-conjugated
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Application
- Western Blotting (WB)
- Purification
- Anti-Anthrax Protective Antigen Antibody is sera. A BLAST analysis was used to suggest cross-reactivity with Anti-Anthrax PA from multiple species based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Anthrax PA from other sources has not been determined.
- Immunogen
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Immunogen: Anthrax Protective Antigen Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the internal region of the Anthrax PA protein.
Immunogen Type: Peptide
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- Application Notes
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Application Note: Anti-Anthrax PA (Protective Antigen) Antibody is suitable for use in WB. Expect a band approximately 83 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:100-1:1000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
0.05 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- Anthrax Protective Antigen (PA)
- Alternative Name
- Anthrax Protective Antigen (PA Products)
- Background
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Synonyms: pag, Anthrax Protective Antigen, Anthrax toxins translocating protein, PA-83, Protective antigen PA-20, PA20, Protective antigen PA-63, PA63
Background: Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxic entry into cells. Anthrax PA is secreted from Bacillus anthracis as an 83 kD (PA83) protein which is cleaved by furin protease, releasing a 20 kD fragment. The 63 kD portion (PA63) of the cleavage product forms a heptamer, which binds the toxins LF and EF. This complex enters via receptor-mediated endocytosis where LF and EF exert their toxic effects. Anthrax PA is ideal for researchers interested in Neurobiology research.
Gene Name: pagA
- Gene ID
- 1158723
- NCBI Accession
- NP_052806
- UniProt
- P13423
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