DMPO antibody (FITC)

Catalog No. ABIN6655017

Supplier Product No.: 200-302-f21

Product Details

Target: DMPO

Reactivity: Adenovirus

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 200-302-f21

Supplier: Rockland

Purpose: DMPO Antibody

Cross-Reactivity (Details): DMPO is species independent.

Purification: Anti-DMPO Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: DMPO Antibody was produced in mice by repeated immunizations with 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.

Clone: N1664A

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-DMPO Antibody is tested for use in WB, IP, IHC and IF microscopy. Expect a band approximately ~90kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Tris Cl, 0.15 M Sodium Chloride, 0.001 M EDTA, pH 7.4

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: DMPO

Target Type: Chemical

Background:

Synonyms: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide

Background: The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.