SPTA1 antibody (AA 356-475)

Catalog No. ABIN6654214

Product Details anti-SPTA1 Antibody

Target: SPTA1 (Spectrin alpha Chain, Erythrocytic 1 (SPTA1))

Binding Specificity: AA 356-475

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SPTA1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A portion of amino acids 356-475 from the human protein was used as the immunogen for the recombinant Spectrin alpha 1 antibody.

Clone: SPTA1-2939R

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant Spectrin alpha 1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Spectrin alpha 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for SPTA1

Target: SPTA1 (Spectrin alpha Chain, Erythrocytic 1 (SPTA1))

Alternative Name: SPTA1 / Spectrin alpha 1 (SPTA1 Products)

Background: Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous and chains, which aggregate side-to-side in an anti-parallel fashion to form dimers, tetramers and higher polymers. Spectrin Ia and spectrin Ib are present in erythrocytes, whereas spectrin IIa (also designated fodrin alpha) and spectrin IIb (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.