CD83 antibody (APC)

Catalog No. ABIN6579823

Product Details anti-CD83 Antibody (APC)

Target: CD83

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD83 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody HB15e recognizes an extracellular epitope of CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human CD83-transfected Cos cells

Clone: HB15e

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for CD83

Target: CD83

Alternative Name: CD83 (CD83 Products)

Synonyms: BL11 antibody, HB15 antibody, CD83 molecule antibody, CD83 antigen antibody, Cd83 antibody, CD83 antibody

Background: CD83 Molecule,CD83 is a 40-45 kDa heavily glycosylated type I cell surface glycoprotein of immunoglobulin family. It is expressed on the surface of mature dendritic cells, Langerhans cells in the skin, and interdigitating reticulum cells in the lymphoid tissues. Low expression of CD83 has been reported in activated T and B cells. Cytoplasmic expression of CD83 can be detected also in monocytes and macrophages. CD83 is involved in modulation of antigen presentation. Soluble CD83 has immunoregulatory functions, it is able to down-regulate dendritic cell maturation and stimulation of T cells. In the developing immune system, release of soluble CD83 from dendritic cells upon stimulation by gram-positive or gram-negative bacteria has anti-allergic effect. Herpes simplex virus, on the other hand, causes CD83 degradation in mature dendritic cells.,BL11, HB15

Gene ID: 9308

UniProt: Q01151