Histone H2B antibody

Catalog No. ABIN6579169

Product Details anti-Histone H2B Antibody

Target: Histone H2B

Reactivity: Saccharomyces cerevisiae, Schizosaccharomyces pombe

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Purification: None

Immunogen: This yeast histone H2B antibody was raised against recombinant yeast histone H2B.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -20 °C

Storage Comment: Antibodies in solution can be stored at -20 °C for 2 years.

Expiry Date: 6 months

References for anti-Histone H2B antibody (ABIN6579169)

Brahma, Henikoff: "RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast." in: Molecular cell, Vol. 73, Issue 2, pp. 238-249.e3, (2019) (PubMed).

Chen, DArcy, Radebaugh, Krzizike, Giebler, Huang, Nyborg, Luger, Stargell: "Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus." in: Molecular and cellular biology, Vol. 36, Issue 8, pp. 1287-96, (2016) (PubMed).

Materne, Vázquez, Sánchez, Yague-Sanz, Anandhakumar, Migeot, Antequera, Hermand: "Histone H2B ubiquitylation represses gametogenesis by opposing RSC-dependent chromatin remodeling at the ste11 master regulator locus." in: eLife, Vol. 5, (2016) (PubMed).

Stulemeijer, De Vos, van Harten, Joshi, Blomberg, van Welsem, Terweij, Vlaming, de Graaf, Altelaar, Bakker, van Leeuwen: "Dot1 histone methyltransferases share a distributive mechanism but have highly diverged catalytic properties." in: Scientific reports, Vol. 5, pp. 9824, (2015) (PubMed).

Zheng, Crickard, Srikanth, Reese: "A highly conserved region within H2B is important for FACT to act on nucleosomes." in: Molecular and cellular biology, Vol. 34, Issue 3, pp. 303-14, (2014) (PubMed).

Bonizec, Hérissant, Pokrzywa, Geng, Wenzel, Howard, Rodriguez, Krause, Tansey, Hoppe, Dargemont: "The ubiquitin-selective chaperone Cdc48/p97 associates with Ubx3 to modulate monoubiquitylation of histone H2B." in: Nucleic acids research, Vol. 42, Issue 17, pp. 10975-86, (2014) (PubMed).

Fleming, Beggs, Church, Tsukihashi, Pennings: "The yeast Cyc8-Tup1 complex cooperates with Hda1p and Rpd3p histone deacetylases to robustly repress transcription of the subtelomeric FLO1 gene." in: Biochimica et biophysica acta, Vol. 1839, Issue 11, pp. 1242-55, (2014) (PubMed).

Bennett, Papamichos-Chronakis, Peterson: "DNA repair choice defines a common pathway for recruitment of chromatin regulators." in: Nature communications, Vol. 4, pp. 2084, (2013) (PubMed).

Rizzardi, Dorn, Strahl, Cook: "DNA replication origin function is promoted by H3K4 di-methylation in Saccharomyces cerevisiae." in: Genetics, Vol. 192, Issue 2, pp. 371-84, (2012) (PubMed).

Samel, Cuomo, Bonaldi, Ehrenhofer-Murray: "Methylation of CenH3 arginine 37 regulates kinetochore integrity and chromosome segregation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 23, pp. 9029-34, (2012) (PubMed).

Moehle, Ryan, Krogan, Kress, Guthrie: "The yeast SR-like protein Npl3 links chromatin modification to mRNA processing." in: PLoS genetics, Vol. 8, Issue 11, pp. e1003101, (2012) (PubMed).

Gardner, Zhou, Parra, Chen, Strahl: "Identification of lysine 37 of histone H2B as a novel site of methylation." in: PLoS ONE, Vol. 6, Issue 1, pp. e16244, (2011) (PubMed).

Chandrasekharan, Huang, Sun: "Decoding the trans-histone crosstalk: methods to analyze H2B ubiquitination, H3 methylation and their regulatory factors." in: Methods (San Diego, Calif.), Vol. 54, Issue 3, pp. 304-14, (2011) (PubMed).

Batta, Zhang, Yen, Goffman, Pugh: "Genome-wide function of H2B ubiquitylation in promoter and genic regions." in: Genes & development, Vol. 25, Issue 21, pp. 2254-65, (2011) (PubMed).

Kyriss, Jin, Gallegos, Sanford, Wyrick: "Novel functional residues in the core domain of histone H2B regulate yeast gene expression and silencing and affect the response to DNA damage." in: Molecular and cellular biology, Vol. 30, Issue 14, pp. 3503-18, (2010) (PubMed).

Nag, Kyriss, Smerdon, Wyrick, Smerdon: "A cassette of N-terminal amino acids of histone H2B are required for efficient cell survival, DNA repair and Swi/Snf binding in UV irradiated yeast." in: Nucleic acids research, Vol. 38, Issue 5, pp. 1450-60, (2010) (PubMed).

Andrews, Chen, Zevin, Stargell, Luger: "The histone chaperone Nap1 promotes nucleosome assembly by eliminating nonnucleosomal histone DNA interactions." in: Molecular cell, Vol. 37, Issue 6, pp. 834-42, (2010) (PubMed).

Target Details for Histone H2B

Target: Histone H2B

Abstract: Histone H2B Products

Synonyms: Hist1h2bc antibody, H2B antibody, HISTONE H2B antibody, MRN17.11 antibody, MRN17_11 antibody, histone B2 antibody, H2B1 antibody, HIS2B antibody, histone cluster 1 H2B family member k antibody, histone H2B antibody, histone B2 antibody, histone H2B.3 antibody, Hist1h2bk antibody, H2B antibody, HTB2 antibody, THAPSDRAFT_27352 antibody, THAPSDRAFT_37309 antibody, THAPS_6947 antibody, THAPSDRAFT_34210 antibody, LOC426037 antibody, LOC107802329 antibody

Molecular Weight: 14 kDa