Estrogen Receptor alpha antibody (AA 246-273)

Catalog No. ABIN657470

Product Details anti-Estrogen Receptor alpha Antibody

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Binding Specificity: AA 246-273

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, Hs, Pig, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ESR1/ER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-273 amino acids from the Central region of human ESR1/ER.

Clone: RB33807

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:2000. WB: 1:2000. IHC-P: 1:100. IHC-P: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: ESR1/ER Antibody (Center) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Expiry Date: 6 months

References for anti-Estrogen Receptor alpha antibody (ABIN657470)

Zhu, Xu, Rashedi, Pavone, Kim, Woodruff, Burdette: "Human fallopian tube epithelium co-culture with murine ovarian follicles reveals crosstalk in the reproductive cycle." in: Molecular human reproduction, Vol. 22, Issue 11, pp. 756-767, (2018) (PubMed).

Wang, Zhou, Guan, Yu, Wang: "β-Estradiol antagonizes the inhibitory effects of caffeine in BMMSCs via the ERβ-mediated cAMP-dependent PKA pathway." in: Toxicology, Vol. 394, pp. 1-10, (2018) (PubMed).

Arslan, Yu, Burdette, Pavone, Hope, Woodruff, Kim: "Novel three dimensional human endocervix cultures respond to 28-day hormone treatment." in: Endocrinology, Vol. 156, Issue 4, pp. 1602-9, (2015) (PubMed).

Bi, Yang, Yu, Zhao, Zhao, He, Jing, Wang, Huang: "Pregnancy serum facilitates hepatitis E virus replication in vitro." in: The Journal of general virology, Vol. 96, Issue Pt 5, pp. 1055-61, (2015) (PubMed).

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1/ER (ESR1 Products)

Background: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.

Molecular Weight: 66216

Gene ID: 2099

NCBI Accession: NP_000116, NP_001116212, NP_001116213, NP_001116214

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly