PLOD1 antibody (N-Term)

Catalog No. ABIN656585

Product Details anti-PLOD1 Antibody

Target: PLOD1 (Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1))

Binding Specificity: AA 66-94, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLOD1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PLOD1.

Clone: RB30211

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. WB: 1:1000-1:2000. WB: 1:2000. IHC-P: 1:25. IHC-P: 1:10~50. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: PLOD1 Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Expiry Date: 6 months

References for anti-PLOD1 antibody (ABIN656585)

Lindert, Cabral, Ausavarat, Tongkobpetch, Ludin, Barnes, Yeetong, Weis, Krabichler, Srichomthong, Makareeva, Janecke, Leikin, Röthlisberger, Rohrbach, Kennerknecht, Eyre, Suphapeetiporn, Giunta et al.: "MBTPS2 mutations cause defective regulated intramembrane proteolysis in X-linked osteogenesis imperfecta. ..." in: Nature communications, Vol. 7, pp. 11920, (2018) (PubMed).

Cabral, Ishikawa, Garten, Makareeva, Sargent, Weis, Barnes, Webb, Shaw, Ala-Kokko, Lacbawan, Högler, Leikin, Blank, Zimmerberg, Eyre, Yamada, Marini: "Absence of the ER Cation Channel TMEM38B/TRIC-B Disrupts Intracellular Calcium Homeostasis and Dysregulates Collagen Synthesis in Recessive Osteogenesis Imperfecta." in: PLoS genetics, Vol. 12, Issue 7, pp. e1006156, (2016) (PubMed).

Wågsäter, Paloschi, Hanemaaijer, Hultenby, Bank, Franco-Cereceda, Lindeman, Eriksson: "Impaired collagen biosynthesis and cross-linking in aorta of patients with bicuspid aortic valve." in: Journal of the American Heart Association, Vol. 2, Issue 1, pp. e000034, (2013) (PubMed).

Target Details for PLOD1

Target: PLOD1 (Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1))

Alternative Name: PLOD1 (PLOD1 Products)

Synonyms: EDS6 antibody, LH antibody, LH1 antibody, LLH antibody, PLOD antibody, Plod antibody, procollagen-lysine antibody, 2410042F05Rik antibody, AI854890 antibody, AV007929 antibody, Lh1 antibody, plod1 antibody, ik:tdsubs_rzpd609-29b19 antibody, plod antibody, wu:fc29c10 antibody, wu:fc95g08 antibody, wu:fi18a05 antibody, zgc:152876 antibody, procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 antibody, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 antibody, procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 antibody, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 L homeolog antibody, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a antibody, PLOD1 antibody, Plod1 antibody, PLOD3 antibody, plod1.L antibody, plod1a antibody

Background: Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.

Molecular Weight: 83550

Gene ID: 5351

NCBI Accession: NP_000293

UniProt: Q02809