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Recombinant BRAF antibody (Val600Glu-Mutant)
BRAF
Reactivity: Human
WB, IHC, ICC
Host: Rabbit
Monoclonal
RM8
unconjugated
Recombinant Antibody
Product Details anti-BRAF Antibody
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Target
See all BRAF Antibodies
BRAF
(B-Raf proto-oncogene, serine/threonine kinase (BRAF))
Antibody Type
Recombinant Antibody
Binding Specificity
All epitopes for BRAF antibodies
Val600Glu-Mutant
Reactivity
All reactivities for BRAF antibodies
Human
Host
All hosts for BRAF antibodies
Rabbit
Clonality
All clonalities for BRAF antibodies
Monoclonal
Conjugate
All conjugates for BRAF antibodies
This BRAF antibody is un-conjugated
Application
All applications for BRAF antibodies
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Characteristics
This antibody reacts to the BRAF V600E mutant. No cross reactivity seen with wild type BRAF.
Purification
Purified
Purity
Protein A purified from animal origin-free supernatant
Immunogen
A peptide corresponding to BRAF V600E mutant was used as the immunogen for the recombinant BRAF V600E antibody.
Clone
RM8
Isotype
IgG
Top Product
Discover our top product BRAF Primary Antibody
Alternatives
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Application Details
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Application Notes
The stated application concentrations are suggested starting points. Titration of the recombinant BRAF V600E antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunohistochemistry: (FFPE): 0.5-5 μg/mL,Immunocytochemistry: 2-10 μg/mL,Western Blot: 0.5-2 μg/mL
Restrictions
For Research Use only
Handling
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Buffer
1 mg/mL in PBS with 50 % Glycerol, 1 % BSA and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Store the recombinant BRAF V600E antibody at -20°C.
Target Details for BRAF
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Target
BRAF
(B-Raf proto-oncogene, serine/threonine kinase (BRAF))
Alternative Name
BRAF V600E (BRAF Products )
Synonyms
B-RAF1 antibody, BRAF1 antibody, NS7 antibody, RAFB1 antibody, C-RMIL antibody, RMIL antibody, B-Raf antibody, braf1 antibody, rafb1 antibody, b-raf1 antibody, sierrabraf antibody, BRAF antibody, 9930012E13Rik antibody, AA120551 antibody, AA387315 antibody, AA473386 antibody, B-raf antibody, Braf-2 antibody, Braf2 antibody, C230098H17 antibody, C87398 antibody, D6Ertd631e antibody, braf antibody, fc48g01 antibody, wu:fc48g01 antibody, B-Raf proto-oncogene, serine/threonine kinase antibody, B-Raf proto-oncogene, serine/threonine kinase S homeolog antibody, Braf transforming gene antibody, si:dkey-222f8.3 antibody, BRAF antibody, Braf antibody, braf.S antibody, braf antibody, si:dkey-222f8.3 antibody
Background
This antibody reacts to the BRAF V600E mutant. No cross reactivity seen with wild type BRAF.
UniProt
P15056
Pathways
MAPK Signaling , RTK Signaling , Neurotrophin Signaling Pathway , Ribonucleoprotein Complex Subunit Organization , Hepatitis C , Autophagy
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