The mouse monoclonal antibody 1A5 recognizes an extracellular epitope of CD170 (Siglec-5, sialic acid binding Ig-like lectin 5), a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. As in case with other anti-CD170 antibodies, this antibody crossreacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Cross-Reactivity (Details)
Human, Non-Human Primates
Purification
Purified by protein-A affinity chromatography.
Purity
> 95 % (by SDS-PAGE)
Immunogen
Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1
Sialic acid binding Ig like lectin 5,CD170, also known as Siglec 5 (sialic acid binding Ig-like lectin 5) is a type 1 transmembrane glycoprotein containing two cytoplasmic immunoreceptor tyrosine inhibitory motifs (ITIMs). CD170 forms homodimers and functions as an inhibitory receptor able to downregulate cell activation. It binds to alpha2,3- and alpha2,6-linked sialic acid ligands, e.g. on glycophorin A (CD235a). Aberrant expression of CD170 by CD34+ progenitor cells can be observed in case of acute myeloid leukemias.,OBBP2, OB-BP2, SIGLEC-5, SIGLEC5