RAD17 antibody (AA 218-246)

Catalog No. ABIN655646

Product Details anti-RAD17 Antibody

Target: RAD17

Binding Specificity: AA 218-246

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD17 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human RAD17.

Clone: RB20035

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for RAD17

Target: RAD17

Alternative Name: RAD17 (RAD17 Products)

Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Molecular Weight: 77055

Gene ID: 5884

NCBI Accession: NP_001265551, NP_002864, NP_579916, NP_579917, NP_579918, NP_579919, NP_579920, NP_579921, NP_579922

UniProt: O75943