CDT2/RAMP antibody (AA 229-256)

Catalog No. ABIN654926

Product Details anti-CDT2/RAMP Antibody

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Binding Specificity: AA 229-256

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDT2/RAMP antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of human DTL.

Clone: RB24420

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for CDT2/RAMP

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Alternative Name: DTL (DTL Products)

Synonyms: CDT2 antibody, DCAF2 antibody, L2DTL antibody, RAMP antibody, 2810047L02Rik antibody, 5730564G15Rik antibody, Ramp antibody, RGD1310439 antibody, cdt2 antibody, cdt2-a antibody, dcaf2 antibody, l2dtl antibody, ramp antibody, cb151 antibody, chunp6871 antibody, wu:fb54b02 antibody, denticleless E3 ubiquitin protein ligase homolog antibody, denticleless E3 ubiquitin protein ligase antibody, denticleless E3 ubiquitin protein ligase homolog S homeolog antibody, denticleless E3 ubiquitin protein ligase homolog (Drosophila) antibody, DTL antibody, Dtl antibody, dtl.S antibody, dtl antibody

Background: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

Molecular Weight: 79468

Gene ID: 51514

NCBI Accession: NP_057532

UniProt: Q9NZJ0