PYM antibody (N-Term)

Catalog No. ABIN654666

Product Details anti-PYM Antibody

Target: PYM (WIBG) (Partner of Y14 and mago (WIBG))

Binding Specificity: AA 12-40, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This WIBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human WIBG.

Clone: RB28144

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for PYM

Target: PYM (WIBG) (Partner of Y14 and mago (WIBG))

Alternative Name: WIBG (WIBG Products)

Synonyms: BEST:HL05757 antibody, CG10330 antibody, CG30176 antibody, Dmel\\CG30176 antibody, PYM antibody, Pym antibody, UD3 antibody, A030010B05Rik antibody, zgc:65891 antibody, zgc:77281 antibody, Partner of Y14 and Mago antibody, Wibg protein antibody, PYM homolog 1, exon junction complex associated factor L homeolog antibody, PYM homolog 1, exon junction complex associated factor antibody, Pym antibody, wibg antibody, pym1.L antibody, PYM1 antibody, Pym1 antibody, pym1 antibody

Background: Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions. May bind RNA, the relevance of RNA-binding remains unclear in vivo, RNA-binding was detected by PubMed:14968132, while PubMed:19410547 did not detect RNA-binding activity independently of the EJC.

Molecular Weight: 22656

Gene ID: 84305

NCBI Accession: NP_001137325, NP_115721

UniProt: Q9BRP8