E2F5 antibody (N-Term)

Catalog No. ABIN652951

Product Details anti-E2F5 Antibody

Target: E2F5

Binding Specificity: AA 66-93, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M, X, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This E2F5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the N-terminal region of human E2F5.

Clone: RB23726

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-E2F5 antibody (ABIN652951)

Song, Gong, Yan, Hong, Wang, Wu, Li, Li, Cao: "Sj7170, a unique dual-function peptide with a specific ?-chymotrypsin inhibitory activity and a potent tumor-activating effect from scorpion venom." in: The Journal of biological chemistry, Vol. 289, Issue 17, pp. 11667-80, (2014) (PubMed).

Target Details for E2F5

Target: E2F5

Alternative Name: E2F5 (E2F5 Products)

Synonyms: AU024671 antibody, E2F-5 antibody, E2f-5 antibody, E2F4 antibody, cb735 antibody, zgc:111879 antibody, E2F transcription factor 5 antibody, E2f5 antibody, e2f5 antibody, E2F5 antibody

Background: E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.

Molecular Weight: 37610

Gene ID: 1875

NCBI Accession: NP_001077057, NP_001077058, NP_001942

UniProt: Q15329