Glutaminase antibody (C-Term)
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- Target See all Glutaminase (GLS) Antibodies
- Glutaminase (GLS)
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Binding Specificity
- AA 516-545, C-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Glutaminase antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Predicted Reactivity
- Rat
- Purification
- This antibody is purified through a protein A column, followed by peptide affinity purification.
- Immunogen
- This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
- Clone
- RB22875
- Isotype
- Ig Fraction
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- Application Notes
- WB: 1:1000. WB: 1:1000. IF: 1:25. IF: 1:25. IF: 1:25. WB: 1:1000. WB: 1:2000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
- Expiry Date
- 6 months
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The oncogenic transcription factor c-Jun regulates glutaminase expression and sensitizes cells to glutaminase-targeted therapy." in: Nature communications, Vol. 7, pp. 11321, (2016) (PubMed).
: "
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The oncogenic transcription factor c-Jun regulates glutaminase expression and sensitizes cells to glutaminase-targeted therapy." in: Nature communications, Vol. 7, pp. 11321, (2016) (PubMed).
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- Target
- Glutaminase (GLS)
- Alternative Name
- GLS (GLS Products)
- Background
- Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.
- Molecular Weight
- 73461
- Gene ID
- 2744
- NCBI Accession
- NP_001243239, NP_055720
- UniProt
- O94925
- Pathways
- Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect
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