Glutaminase antibody (C-Term)

Catalog No. ABIN652757

Product Details anti-Glutaminase Antibody

Target: Glutaminase (GLS)

Binding Specificity: AA 516-545, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.

Clone: RB22875

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IF: 1:25. IF: 1:25. IF: 1:25. WB: 1:1000. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-Glutaminase antibody (ABIN652757)

Lukey, Greene, Erickson, Wilson, Cerione: "The oncogenic transcription factor c-Jun regulates glutaminase expression and sensitizes cells to glutaminase-targeted therapy." in: Nature communications, Vol. 7, pp. 11321, (2016) (PubMed).

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: GLS (GLS Products)

Background: Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.

Molecular Weight: 73461

Gene ID: 2744

NCBI Accession: NP_001243239, NP_055720

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect